Immunomodulatory and immunocorrective therapies are important approaches to foster immunity. Biologically active substances (BAS) of plants, exhibiting a wide spectrum of pharmacological activity, can act as agents mobilizing the immune system. Plants belonging to the Caryophyllaceae family, including Silene sendtneri and S. roemeri, are rich in ecdysteroids and flavonoids, the compounds characterized by a large variety of biological activities with potential impact on the human immune system. In this study, the effect of native and chlorophyll-free plant extracts of S. sendtneri and S. roemeri on human monocyte-derived macrophages was examined. The production of cytokines was assessed using ELISA. Gene expression microarrays and RT-qPCR analysis were used to determine differentially expressed genes under the influence of the extracts. The results of the experiments showed that the extracts increased production of an M2-associated chemokine CCL-18 in macrophages. In addition, they induced the production of proinflammatory cytokines (TNF-α, IL-1β, and IL-6). The identified activity was detected both in native and chlorophyll-free extracts. Furthermore, plant extracts of S. sendtneri and S. roemeri influenced the expression of the CCL2, MMP9, VEGFA, and S100A9 genes in human macrophages. Overall, extracts of the Silene genus plants showed immunomodulatory effects by influencing macrophage activation in vitro.

中文翻译：

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Silene roemeri 和 Silene sendtneri 乙醇提取物对人单核细胞/巨噬细胞的影响评估

免疫调节和免疫矫正疗法是增强免疫力的重要方法。植物的生物活性物质 (BAS) 具有广泛的药理活性，可以作为动员免疫系统的药剂。属于石竹科的植物，包括 Silene sendtneri 和 S. roemeri，富含蜕皮甾类和黄酮类化合物，这些化合物具有多种生物活性，对人体免疫系统有潜在影响。在这项研究中，检查了 S. sendtneri 和 S. roemeri 的天然和不含叶绿素的植物提取物对人单核细胞衍生的巨噬细胞的影响。使用ELISA评估细胞因子的产生。基因表达微阵列和 RT-qPCR 分析用于确定提取物影响下的差异表达基因。实验结果表明，提取物增加了巨噬细胞中 M2 相关趋化因子 CCL-18 的产生。此外，它们还诱导了促炎细胞因子（TNF-α、IL-1β 和 IL-6）的产生。在天然和不含叶绿素的提取物中均检测到鉴定的活性。此外，S. sendtneri 和 S. roemeri 的植物提取物影响 CCL2、MMP9、VEGFA 和 S100A9 基因在人类巨噬细胞中的表达。总体而言，Silene 属植物的提取物通过影响体外巨噬细胞活化显示出免疫调节作用。在天然和不含叶绿素的提取物中均检测到鉴定的活性。此外，S. sendtneri 和 S. roemeri 的植物提取物影响 CCL2、MMP9、VEGFA 和 S100A9 基因在人类巨噬细胞中的表达。总体而言，Silene 属植物的提取物通过影响体外巨噬细胞活化显示出免疫调节作用。在天然和不含叶绿素的提取物中均检测到鉴定的活性。此外，S. sendtneri 和 S. roemeri 的植物提取物影响 CCL2、MMP9、VEGFA 和 S100A9 基因在人类巨噬细胞中的表达。总体而言，Silene 属植物的提取物通过影响体外巨噬细胞活化显示出免疫调节作用。