Abstract — In order to develop a target drug we studied the process of conjugation of recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) and alendronic acid (ALN). The conjugation reactions were performed both in solution and in the solid phase. The results of the examination of the specific activity of the conjugated GM-CSF confirmed the preservation of biological properties of the protein. The experimental data obtained indicate a higher affinity of GM-CSF-ALN conjugates to hydroxyapatite, an analog of the bone mineral matrix, than of the intact protein.

中文翻译：

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阿仑膦酸合成GM-CSF偶联物方法的研究

摘要 — 为了开发靶向药物，我们研究了重组人粒细胞-巨噬细胞集落刺激因子（GM-CSF）和阿仑膦酸（ALN）的结合过程。结合反应在溶液和固相中进行。对结合的 GM-CSF 的比活性的检查结果证实了蛋白质的生物学特性的保留。获得的实验数据表明，与完整蛋白质相比，GM-CSF-ALN 缀合物对羟基磷灰石（骨矿物质基质的类似物）的亲和力更高。