Recently, researchers have reported the presence of patulin as a mycotoxin in commercial apple products, especially apple juices. The aim of this study was to assess adsorption of patulin from artificially contaminated apple juice using two lactic acid bacteria (LAB) strains of Lactobacillus acidophilus ATCC 4356 and Lactobacillus plantarum ATCC 8014. Furthermore, effects of five physical and chemical pretreatments on the patulin adsorption were investigated. Results demonstrated that patulin adsorption abilities of both strains increased with NaOH pretreatment but decreased after autoclaving. The NaOH-treated L. plantarum ATCC 8014 showed the best removal rate (59.74%) after 48 h of refrigerated storage, compared with the NaOH-treated L. acidophilus ATCC 4356 (52.36%). Moreover, stability of the LAB-patulin complex was assessed in simulated gastrointestinal tract conditions and a low quantity of patulin was released into the solution. The patulin adsorption process by NaOH-treated L. plantarum ATCC 8014 followed Freundlich isotherm model and pseudo-second-order kinetic model. Fourier transform infrared spectroscopy showed that polysaccharide and protein components of the L. plantarum ATCC 8014 cell wall played key roles in patulin adsorption. The major functional groups of the cell wall that were involved in adsorbing patulin included -OH/-NH, -CH₂, C=O, and C-O groups. The current results suggest that NaOH-treated L. plantarum ATCC 8014 cells include the potential to detoxify patulin-contaminated apple juices.

最近，研究人员报告了在商业苹果产品（尤其是苹果汁）中存在棒曲霉素作为霉菌毒素的一种毒素。这项研究的目的是评估使用两种乳酸菌（LAB）嗜酸乳杆菌ATCC 4356和植物乳杆菌ATCC 8014从人工污染的苹果汁中提取的棒曲霉素的吸附。此外，五种物理和化学预处理对棒曲霉素的吸附作用为调查。结果表明，两种菌株的棒曲霉素吸附能力均通过NaOH预处理增加，但在高压灭菌后降低。经NaOH处理的植物乳杆菌ATCC 8014与经NaOH处理的植物相比，冷藏后48小时显示出最佳去除率（59.74％）嗜酸乳杆菌ATCC 4356（52.36％）。此外，在模拟胃肠道条件下评估了LAB-patulin复合物的稳定性，并向溶液中释放了少量的patulin。NaOH处理过的植物乳杆菌ATCC 8014对棒曲霉素的吸附过程遵循Freundlich等温模型和伪二级动力学模型。傅里叶变换红外光谱表明，植物乳杆菌ATCC 8014细胞壁的多糖和蛋白质成分在棒曲霉素的吸附中起关键作用。细胞壁中参与棒曲蛋白吸附的主要官能团包括-OH / -NH，-CH ₂，C = O和CO。目前的结果表明，NaOH处理过的植物乳杆菌 ATCC 8014细胞具有排解受Patulin污染的苹果汁的潜力。