Phragmites australis grows as a pioneer plant species in several mine and flotation tailings ponds distinguished by extremely high concentrations of metals. The main goals of this study were to estimate the effects of the specific concentrations and combinations of accumulated metals on the efficiency of antioxidative enzymes and plant oxidative status. This study is relevant to our understanding of the common reed exceptional capacity to endure extreme edaphic conditions. Metal concentrations were determined in the sediment, roots and leaves. Antioxidative enzymes activities, amounts of pigments and phenolics, total antioxidative capacity (TAC), lipid peroxidation level (LP) were analysed in plant organs. Effects of accumulated metals depended on their concentrations and their stoichiometry. Antioxidative enzymes and TAC in roots were significantly reduced, resulting in consequent increase in LP. Pb concentration in leaves did not significantly change enzymes activities, whereas toxic level of Cu impeded activity of catalaze and ascorbate peroxidase. The results indicate that in the conditions of high root metal contamination the mechanisms involved in their immobilization and detoxification cannot completely restrain their toxicity. Their effects on enzymes activities depend on the type of enzyme, metal concentrations, specific ratios and interactions.

中文翻译：

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高金属浓度对矿山和浮选尾矿池芦苇抗氧化系统的影响

芦苇作为先驱植物物种生长在几个以极高浓度金属为特征的矿山和浮选尾矿池中。本研究的主要目标是评估特定浓度和累积金属组合对抗氧化酶的效率和植物氧化状态的影响。这项研究与我们对普通芦苇耐受极端土壤条件的特殊能力的理解有关。在沉积物、根和叶中测定金属浓度。在植物器官中分析抗氧化酶活性、色素和酚类物质的量、总抗氧化能力（TAC）、脂质过氧化水平（LP）。累积金属的影响取决于它们的浓度和化学计量。根中的抗氧化酶和 TAC 显着降低，导致 LP 随之增加。叶子中的铅浓度没有显着改变酶的活性，而铜的毒性水平阻碍了过氧化氢酶和抗坏血酸过氧化物酶的活性。结果表明，在高根金属污染条件下，其固定和解毒机制不能完全抑制其毒性。它们对酶活性的影响取决于酶的类型、金属浓度、特定比例和相互作用。结果表明，在高根金属污染条件下，其固定和解毒机制不能完全抑制其毒性。它们对酶活性的影响取决于酶的类型、金属浓度、特定比例和相互作用。结果表明，在高根金属污染条件下，其固定和解毒机制不能完全抑制其毒性。它们对酶活性的影响取决于酶的类型、金属浓度、特定比例和相互作用。