Differential effects of the seed exudates of susceptible and resistant soybean cultivars Sloan and Williams 82 on the zoospores of soilborne pathogenic Phytophthora sojae are not clear. This study aims to elucidate the role of the seed exudates in the host resistance of soybean against P. sojae during prior to infection. The response behaviors of P. sojae zoospores to the seed exudates were performed in an assay chamber and concave slide, the proteomes of P. sojae zoospores treated with the seed exudates were analyzed by TMT method. The expression of genes encoding key proteins was quantified by qRT-PCR. The zoospores of P. sojae exhibited significant chemotaxis to the both seed exudates and a stronger attracted was found to the seed exudates of Sloan. The seed exudates of Sloan also had a stonger promotion than that of Williams 82 on encystment of zoospores. During the responding process, the key proteins in zoospores related to chemotaxis were upregulated, the upregulation fold of these proteins in responding to Sloan was higher than that of Williams 82. Proteins differentially expressed promote the encystment of zoospores. The changes in expression of these proteins was several fold higher in the zoosprores exposed to the seed exudates of Sloan than that of Williams 82. Evidence of differential changes in protein expression in P. sojae zoospores exposed to the seed exudates of both soybean supports the differential developmental behaviors such as chemotaxis and encystment of the zoospores in responding to the two seed exudates.

中文翻译：

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大豆疫霉游动孢子对大豆种子分泌物的差异反应提供了种子分泌物参与宿主抗性的证据

敏感和抗性大豆品种 Sloan 和 Williams 82 的种子分泌物对土传致病大豆疫霉游动孢子的差异影响尚不清楚。本研究旨在阐明种子分泌物在感染前大豆对大豆疫霉宿主抗性中的作用。大豆疫霉游动孢子对种子分泌物的响应行为在化验室和凹面载玻片中进行，用TMT方法分析大豆疫霉游动孢子的蛋白质组。通过qRT-PCR定量编码关键蛋白的基因的表达。P. sojae 的游动孢子对两种种子分泌物都表现出显着的趋化性，并且发现对 Sloan 的种子分泌物有更强的吸引力。Sloan 的种子分泌物在游动孢子的成囊方面也比 Williams 82 的种子分泌物有更大的促进作用。在响应过程中，游动孢子中与趋化性相关的关键蛋白质上调，这些蛋白质响应Sloan的上调倍数高于Williams 82。差异表达的蛋白质促进了游动孢子的成囊。这些蛋白质在暴露于 Sloan 种子分泌物的游动孢子中的表达变化是 Williams 82 的数倍。暴露于两种大豆种子分泌物的大豆疫霉游动孢子中蛋白质表达的差异变化的证据支持差异发育行为，例如游动孢子对两种种子分泌物的趋化性和包囊反应。游动孢子中与趋化性相关的关键蛋白质上调，这些蛋白质响应Sloan的上调倍数高于Williams 82。差异表达的蛋白质促进了游动孢子的成囊。这些蛋白质在暴露于 Sloan 种子分泌物的游动孢子中的表达变化是 Williams 82 的数倍。暴露于两种大豆种子分泌物的大豆疫霉游动孢子中蛋白质表达的差异变化的证据支持差异发育行为，例如游动孢子对两种种子分泌物的趋化性和包囊反应。游动孢子中与趋化性相关的关键蛋白质上调，这些蛋白质响应Sloan的上调倍数高于Williams 82。差异表达的蛋白质促进了游动孢子的成囊。这些蛋白质在暴露于 Sloan 种子分泌物的游动孢子中的表达变化是 Williams 82 的数倍。暴露于两种大豆种子分泌物的大豆疫霉游动孢子中蛋白质表达的差异变化的证据支持差异发育行为，例如游动孢子对两种种子分泌物的趋化性和包囊反应。差异表达的蛋白质促进游动孢子的成囊。这些蛋白质在暴露于 Sloan 种子分泌物的游动孢子中的表达变化是 Williams 82 的数倍。暴露于两种大豆种子分泌物的大豆疫霉游动孢子中蛋白质表达的差异变化的证据支持差异发育行为，例如游动孢子对两种种子分泌物的趋化性和包囊反应。差异表达的蛋白质促进游动孢子的成囊。这些蛋白质在暴露于 Sloan 种子分泌物的游动孢子中的表达变化是 Williams 82 的数倍。暴露于两种大豆种子分泌物的大豆疫霉游动孢子中蛋白质表达的差异变化的证据支持差异发育行为，例如游动孢子对两种种子分泌物的趋化性和包囊反应。