Ceropegia media is an endemic and endangered plant as its propagation through seeds is unreliable due to low germination, slow growth and seedling decay under natural conditions. Also, tubers of this plant are edible serving as carbohydrate source with medicinal values leading to severe population decline in the natural habitat. To provide a sustainable solution, an efficient in vitro propagation protocol along with phytochemical profiling was developed for C. media. Callus cultures were induced from seedling and wild leaf tissues using the most effective Murashige and Skoog’s (MS) medium with 2,4-dichlorophenoxyacetic acid (2,4-D; 2 µM) and sucrose (3%). Somatic embryos were acquired on MS medium with 1 µM 6-Benzylaminopurine (BAP) and 1 µM 2,4-D. Conversion into plantlets was attained only from tissue culture-derived seedling leaf (TCDSL) explant. Further, in vitro tuberization was achieved from TCDSL callus with BAP and Naphthalene acetic acid (NAA). AgNO3 as an elicitor had a positive effect on both fresh and dry weights of callus. Successful acclimatization (58%) was attained after two months resulting in normal phenotype in pots. Further, metabolite profiles of ten different tissues from wild and in vitro plants were compared. Total 82 compounds comprising alkaloids, fatty acids, fatty acid ester, steroids, terpenes and hydrocarbons were identified. Overall, results suggested enhanced production of selected metabolites with in vitro propagation and AgNO3, alleviating the problem of unavailability of planting materials. Thus, the current study might offer potential ways for the conservation of such RED enlisted species as C. media.

中文翻译：

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通过体外再生和 Ceropegia media (Huber) Ans. 的植物化学分析的组合方法：保护印度西高止山脉濒危药用植物的潜在途径

Ceropegia media 是一种地方性和濒临灭绝的植物，因为在自然条件下，由于发芽率低、生长缓慢和幼苗腐烂，其通过种子繁殖是不可靠的。此外，这种植物的块茎是可食用的，作为具有药用价值的碳水化合物来源，导致自然栖息地的人口严重下降。为了提供可持续的解决方案，为 C. 培养基开发了一种有效的体外繁殖协议以及植物化学分析。使用含有 2,4-二氯苯氧乙酸 (2,4-D; 2 µM) 和蔗糖 (3%) 的最有效 Murashige 和 Skoog's (MS) 培养基从幼苗和野生叶组织中诱导愈伤组织培养物。体细胞胚胎是在含有 1 µM 6-Benzylaminopurine (BAP) 和 1 µM 2,4-D 的 MS 培养基上获得的。仅从组织培养衍生的幼苗叶 (TCDSL) 外植体转化为小植株。此外，用 BAP 和萘乙酸 (NAA) 从 TCDSL 愈伤组织实现了体外块茎化。AgNO3 作为诱导剂对愈伤组织的鲜重和干重均有积极影响。两个月后成功驯化 (58%)，导致盆栽正常表型。此外，还比较了来自野生和体外植物的十种不同组织的代谢物谱。共鉴定出 82 种化合物，包括生物碱、脂肪酸、脂肪酸酯、类固醇、萜烯和碳氢化合物。总体而言，结果表明通过体外繁殖和 AgNO3 提高了选定代谢物的产量，缓解了种植材料不可用的问题。因此，