In this study, we sequenced two complete mitochondrial genomes of Amblyomma ovale, a tick of public health importance. Sequencing two distinct individuals, the resulting mitochondrial genomes were 14,756 and 14,760 bp in length and maintained the same gene order previously reported in Amblyomma. These were combined with RNA-seq derived mitochondrial sequences from three additional species, Amblyomma aureolatum, Amblyomma maculatum, and Amblyomma moreliae, to carry out mitogenome comparative and evolutionary analyses against all previously published tick mitochondrial genomes. We described a derivative genome rearrangement that isolates Ixodes from the remaining Ixodidae and consists of both a reverse translocation as well as an event of Tandem Duplication Random Loss. Genetic distance analyses indicated that cox2, nd1, nd5, and 16S are good candidates for future population studies in A. ovale. The phylogenetic analyses corroborated the utility of complete mitochondrial genomes as phylogenetic markers within the group. This study further supplements the genome information available for Amblyomma and facilitates future evolutionary and population genetic studies within the genus.

中文翻译：

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卵形盲肠线粒体完整基因组的表征，比较分析和系统发育考虑。

在这项研究中，我们对卵圆无形盲mb的两个完整的线粒体基因组进行了测序，这对公共卫生至关重要。测序两个不同的个体，将得到的线粒体基因组是14756且长度为14760 bp和保持先前所报告的相同的基因顺序花蜱。将这些与来自其他三个物种RNA seq的线粒体序列相结合，分别是金黄色盲肠杆菌，黄斑盲肠杆菌和莫里姆盲肠，对所有先前发表的壁虱线粒体基因组进行线粒体基因组比较和进化分析。我们描述了分离异形体的衍生基因组重排来自其余的x科，包括反向易位以及串联复制随机丢失事件。遗传距离分析表明，cox2，nd1，nd5和16S是今后对卵圆线虫种群研究的很好的候选对象。系统发育分析证实了完整的线粒体基因组作为该组内系统发育标记的效用。这项研究进一步补充了可用于盲肠的基因组信息，并促进了该属未来的进化和种群遗传学研究。