Low survival rate of grafted mesenchymal stem cells (MSC) in injured tissue is one of the major limitations of stem cell therapy. One of the most important factors that limits the MSCs survival rate and retention is ischemic stress, which can lead to damage to all components of the cell. In particular, it can damage mitochondria, that play an important role in apoptosis with releasing apoptotic factors. Therefore, we investigated the protective effects of Acetyl-l-carnitine (ALCAR) against serum and glucose deprivation (SGD) in adipose-derived mesenchymal stem cells (AD-MSCs). We measured cell viability, proliferation, and apoptosis in cells experiencing SGD stress for 8 h with exposure to varying concentrations of ALCAR. Results showed that ALCAR protects cells against SGD stress by reducing apoptosis. Its protective effects are associated with reductions in cleaved caspase-3 and attenuation of apoptosis. Result showed that ALCAR exhibits protective effects against SGD-induced damage to AD-MSCs by enhancing the expression of survival signals and by decreasing the expression of death signals.

受损组织中移植间充质干细胞 (MSC) 的低存活率是干细胞治疗的主要限制之一。限制 MSCs 存活率和保留率的最重要因素之一是缺血应激，它会导致细胞的所有成分受损。特别是，它可以破坏线粒体，线粒体在细胞凋亡中发挥重要作用，释放凋亡因子。因此，我们研究了 Acetyl- l的保护作用。-肉碱 (ALCAR) 对抗脂肪来源间充质干细胞 (AD-MSC) 中的血清和葡萄糖剥夺 (SGD)。我们在暴露于不同浓度的 ALCAR 的情况下测量了经历 SGD 应激 8 小时的细胞的细胞活力、增殖和凋亡。结果表明，ALCAR 通过减少细胞凋亡来保护细胞免受 SGD 应激。其保护作用与裂解的 caspase-3 减少和细胞凋亡减弱有关。结果表明，ALCAR通过增强存活信号的表达和降低死亡信号的表达，对SGD诱导的AD-MSCs损伤具有保护作用。