Tissue culture of Berula erecta is suitable cultivation system for research purposes connected with contamination and phytoremediation studies. In previous investigation we determined the optimal dose concentration at which Se stimulates plant growth and positively affects the antioxidative status in this experimental system. In current study, we investigate its response to exposure to lead (Pb) and further the possible protective effect of Se(IV) against Pb exposure. Plants were grown in 10 and 50 mg Pb L⁻¹ solution without and with added Se (0.1 mg L⁻¹) for six weeks. Plants possessed a high affinity to uptake Pb and Se in roots. Addition of Pb inhibited roots elongations and the plant height. In contrast, the combined effect of Se + Pb treatment was reflected in increased weight of plants when compared to Pb treatment alone. Pb decreased the amount of chlorophylls and consequently photochemical efficiency was lowered, whereas in Pb + Se treatment the photochemical efficiency was higher. Furthermore, Pb treatment caused a gradual increase in glutathione in both roots and shoots, however, to a greater percentage in shoots when compared to controls. Exposure to both Pb and Se did not cause any significant changes in root’s glutathione level when compared to Pb treatment alone. In shoots, the combined treatment lowered the glutathione significantly, but the levels remained 50% above those of untreated control samples, reflecting that this might be related with the antioxidative effects of Se treatment.

中文翻译：

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直立铍对硒与硒的反应。

Berula erecta的组织培养是适合与污染和植物修复研究相关的研究目的的培养系统。在先前的研究中，我们确定了硒刺激植物生长并积极影响该实验系统中抗氧化状态的最佳剂量浓度。在当前的研究中，我们调查了其对铅（Pb）暴露的反应，以及硒（IV）对铅暴露的可能的保护作用。植物在10和50 mg Pb L ^-1溶液中生长，不添加和添加Se（0.1 mg L ^-1）的六个星期。植物对根部吸收Pb和Se具有很高的亲和力。铅的添加抑制了根的伸长和株高。相反，与单独的Pb处理相比，Se + Pb处理的综合效果反映在植物重量的增加上。Pb减少了叶绿素的含量，因此降低了光化学效率，而在Pb + Se处理中，光化学效率更高。此外，Pb处理导致根和茎中的谷胱甘肽逐渐增加，但是与对照相比，芽中的谷胱甘肽含量更高。与单独使用铅处理相比，暴露于铅和硒均不会引起根的谷胱甘肽水平发生任何显着变化。在芽中，联合处理可显着降低谷胱甘肽，