Due to the increasingly heated debate on the potential threat of genetically modified (GM) crops to human health and environment, regulations and laws relevant to GM crops have been issued in many countries and regions to strictly restrict their cultivation and application. Therefore, fast and accurate method to realized on-site detection of GM crops is greatly demanded. In this work, a novel isothermal amplification method termed denaturation bubble-mediated strand exchange amplification (SEA) was proposed first time to detect GM crops. Fluorescence assay based on SEA could accurately distinguished GM and non-GM soybean by detecting agrobacterium tumefaciens nopaline synthase (NOS) terminator, which was widely incorporated in GM crops. Moreover, this feasible and specific method could detect NOS terminator from as low as 200 pg/μL total genomic DNA of GM soybean. In addition, in the actual sample detection, the result of colorimetric assay based on SEA results could be directly observed by the naked eyes within 58 min. Compared with the traditional methods based on PCR, which normally required complex equipment, skilled technicians and long operation time, this simple, fast and precise method is more desirable for the on-site GM crops detection.

由于关于转基因作物对人类健康和环境的潜在威胁的辩论日益激烈，许多国家和地区已经发布了与转基因作物有关的法规和法律，以严格限制其种植和应用。因此，迫切需要一种快速，准确的方法来实现转基因作物的现场检测。在这项工作中，首次提出了一种新的等温扩增方法，称为变性气泡介导的链交换扩增（SEA），以检测转基因作物。基于SEA的荧光检测可以通过检测广泛应用于转基因作物中的根癌农杆菌胭脂碱合酶（NOS）终止子来准确区分转基因和非转基因大豆。此外，这种可行且特定的方法可以从低至200 pg /μL的转基因大豆总基因组DNA中检测NOS终止子。另外，在实际样品检测中，可以在58分钟内用肉眼直接观察到基于SEA结果的比色测定结果。与通常需要复杂的设备，熟练的技术人员和较长的操作时间的基于PCR的传统方法相比，这种简单，快速，精确的方法对于现场转基因作物的检测更为理想。