当前位置: X-MOL 学术Nanoscale › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
DNA density-dependent uptake of DNA origami-based two-or three-dimensional nanostructures by immune cells.
Nanoscale ( IF 6.7 ) Pub Date : 2020-06-19 , DOI: 10.1039/d0nr02361b
Tatsuoki Maezawa 1 , Shozo Ohtsuki 1 , Kumi Hidaka 2 , Hiroshi Sugiyama 3 , Masayuki Endo 3 , Yuki Takahashi 1 , Yoshinobu Takakura 1 , Makiya Nishikawa 4
Affiliation  

DNA nanostructures are expected to be applied for targeted drug delivery to immune cells. However, the structural properties of DNA nanostructures required for the delivery have not fully been elucidated. In this study, we focused on the DNA density that can be important for the their recognition and uptake by immune cells. To examine this, DNA nanostructures with almost identical molecular weights and structural flexibility, but with different shapes and DNA densities, were designed using DNA origami technology. We compared the following five types of DNA nanostructures, all of which consisted of ten DNA helices using an identical circular, single-stranded scaffold and staples. Rec180 had a rectangular-shaped, almost flat structure. Rec90, Rec50 and Rec0 were bent forms of Rec180 at the center by 90, 50 or 0 degrees, respectively. Rec50/50 has two bends of 50 degrees each so that the both ends stick together to form a triangular prism shape. The fluctuation, or flexibility, of these DNA nanostructures under solution conditions was estimated using CanDo software. The DNA density estimated from the average distance between any two of the ten DNA helices in the DNA nanostructures was different among them; Rec50, Rec0 and Rec50/50 had a higher density than Rec180 and Rec90. Agarose gel electrophoresis and atomic force microscopy showed that all of the nanostructures were prepared with high yield. Flow cytometry analysis revealed that the uptake of DNA nanostructures by murine macrophage-like RAW264.7 cells was higher for those with higher DNA density than those with low density. There was a positive correlation between the density and cellular uptake. These results indicate that DNA nanostructures with high DNA density are suitable for delivery to immune cells.

中文翻译:

DNA密度依赖免疫细胞对基于DNA折纸的二维或三维纳米结构的吸收。

DNA纳米结构有望用于靶向药物向免疫细胞的递送。然而,尚未完全阐明递送所需的DNA纳米结构的结构特性。在这项研究中,我们集中于DNA密度,这对于免疫细胞对其的识别和摄取可能很重要。为了检验这一点,使用DNA折纸技术设计了具有几乎相同的分子量和结构柔性,但是具有不同形状和DNA密度的DNA纳米结构。我们比较了以下五种类型的DNA纳米结构,它们全部由十个DNA螺旋组成,它们使用相同的圆形,单链支架和钉书钉。Rec180具有矩形的几乎平坦的结构。Rec90,Rec50和Rec0在中心分别是Rec180的弯曲形式,分别为90度,50度或0度。Rec50 / 50具有两个分别为50度的弯曲,因此两端都粘在一起以形成三棱柱形状。使用CanDo软件估算了溶液条件下这些DNA纳米结构的波动或柔韧性。根据DNA纳米结构中十个DNA螺旋中任意两个螺旋之间的平均距离估算的DNA密度在它们之间是不同的。Rec50,Rec0和Rec50 / 50具有比Rec180和Rec90高的密度。琼脂糖凝胶电泳和原子力显微镜观察表明,所有纳米结构均以高收率制备。流式细胞仪分析表明,鼠类巨噬细胞样RAW264.7细胞对DNA密度较高的细胞的摄取要比低密度的细胞高。密度与细胞摄取之间呈正相关。
更新日期:2020-07-16
down
wechat
bug