Fibrinogen (FIB) plays a key role in blood coagulation and thrombosis and its concentration in blood can directly reflect health conditions, thus efficient detection of FIB would benefit the treatments of certain diseases such as liver and heart diseases. However, there is a lack of sensitive, simple, rapid and cheap FIB detection device currently, in lieu of expensive and sophisticated approaches in laboratories. Here, we propose a novel plasma separation and FIB detection platform based on a microfluidic paper-based analytical device (μPAD). It is the first time that dielectrophoretic (DEP) force is combined with capillary force on paper for plasma separation, and the separation efficiency of plasma reaches about 95%, ensuring reliable downstream FIB detection, for which we also propose a new method called the resistance-fibrinogen detection (RFD) method. It not only avoids the use of large-scale instruments for detection, but also possesses high precision and simplicity. The method is found to be reliable in FIB detection for various concentrations ranging from 127.0 to 508.0 mg dL⁻¹. Moreover, the results obtained from the proposed μPAD show an excellent agreement (R² = 0.9985) with those obtained from an automatic coagulation analyzer with natural human blood samples. Overall, the proposed platform provides a low-cost and reliable approach for FIB detection, especially for clinical use in resource-limited areas.

中文翻译：

---

在基于微流纸的分析设​​备上进行纤维蛋白原定量的集成平台。

纤维蛋白原（FIB）在凝血和血栓形成中起着关键作用，其在血液中的浓度可以直接反映健康状况，因此有效检测FIB将有益于某些疾病的治疗，例如肝脏和心脏病。然而，目前缺乏灵敏，简单，快速和廉价的FIB检测装置来代替实验室中的昂贵和复杂的方法。在这里，我们提出了一种基于微流体纸质分析设备（μPAD）的新型血浆分离和FIB检测平台。这是首次将纸上的介电泳力（DEP）力与毛细力结合起来用于血浆分离，血浆分离效率达到约95％，从而确保可靠的下游FIB检测，为此，我们还提出了一种称为抗纤维蛋白原检测（RFD）方法的新方法。它不仅避免了使用大型仪器进行检测，而且具有高精度和简便性。发现该方法对于127.0至508.0 mg dL的各种浓度的FIB检测都是可靠的^-1。此外，从建议的μPAD获得的结果与从自动凝血分析仪获得的具有自然人血样品的结果显示出极好的一致性（ R ² = 0.9985）。总体而言，该平台为FIB检测提供了一种低成本且可靠的方法，尤其是在资源有限的地区用于临床。