Abstract The biofilm formation on food-contact-surfaces is a serious threat for public health and often results in huge human and economic losses. Thus, it is of importance to develop new strategies that allow the monitoring of the biofilm formation on food-contact-surfaces. Such strategies permit effective and timely countermeasures and, therefore, to avoid the microbiological risk and associated environmental impact due to cleaning processes. Therefore, this work investigated a non-invasive method for monitoring and detection of the biofilm formation using Coda Wave Interferometry (CWI) method. The principle of this method and the calculation of decorrelation coefficient based on the ultrasonic measurements were explained. The results underlined that our developed method is able to detect the early stage of the biofilm formation of Staphylococcus aureus on the stainless steel. Namely, it is shown that the bacterial cell kinetic is well captured by the evolution of the decorrelation coefficient as a function of incubation times. Overall, this work showed that this cost effective CWI method is a promising tool for the detection of early stage biofilm formation on food-contact-surfaces.

中文翻译：

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超声波尾波干涉法检测生物膜形成

摘要 食品接触表面形成的生物膜对公众健康构成严重威胁，往往会造成巨大的人员和经济损失。因此，开发新的策略以监测食品接触表面的生物膜形成是很重要的。这样的策略允许有效和及时的对策，因此避免由于清洁过程引起的微生物风险和相关的环境影响。因此，这项工作研究了一种使用 Coda Wave Interferometry (CWI) 方法监测和检测生物膜形成的非侵入性方法。阐述了该方法的原理和基于超声测量的去相关系数的计算。结果强调，我们开发的方法能够检测不锈钢上金黄色葡萄球菌生物膜形成的早期阶段。即，显示细菌细胞动力学被作为孵育时间函数​​的去相关系数的演变很好地捕获。总体而言，这项工作表明，这种具有成本效益的 CWI 方法是检测食品接触表面早期生物膜形成的有前途的工具。