European Journal of Protistology ( IF 2.9 ) Pub Date : 2020-06-19 , DOI: 10.1016/j.ejop.2020.125726 Ashutosh Panda 1 , Bijay Ranjan Mirdha 1 , Neha Rastogi 1 , Samander Kasuhik 2
The present study is an attempt to investigate the presence of Naegleria fowleri in Indian population. A total of 307 patients were enrolled and water samples were collected from both residential and surrounding areas of patients found positive for N. fowleri. The different species of Naegleria from both clinical and water samples were identified taxonomically. Recommended microbiological conventional techniques were used to identify different Naegleria stages and other free-living amoebae from the samples. PCR assays, using both genus and species specific primers were also optimized. None of the samples were positive by conventional microbiological examinations. However, PCR assays detected only three samples positive for N. fowleri. A total of 10 water bodies (ponds), that were used by Naegleria positive patients were examined. The pH and temperature of the water samples collected from water bodies ranged between 5.6–7.2 and 25–32 °C respectively. Among all the 10 water samples tested, four samples were positive for genus Naegleria by PCR assay, of which only two samples, showed positive amplification for N. fowleri. The sequence analysis of N. fowleri strain belonged to genotype II.
中文翻译:
了解印度北部各州患者的“ Naegleria fowleri”(Vahlkampfiidae)的真正负担:来源追踪和意义。
本研究是试图调查印度洋人群中Naegleria fowleri的存在。总共有307名患者参加,并从住宅采集水样和患者的周边地区发现的阳性N.阿米巴。从临床和水样本中分类识别出不同种类的Naegleria。使用推荐的微生物常规技术从样品中鉴定出不同的Naegleria阶段和其他自由活动的变形虫。还优化了使用属和种特异性引物的PCR测定。通过常规微生物学检查,所有样品均未呈阳性。但是,PCR检测仅检测到3份阳性的样品福勒里猪笼草。总共检查了Naegleria阳性患者使用的10个水体(池)。从水体收集的水样的pH和温度分别在5.6–7.2和25–32°C之间。在所测试的所有10个水样品中,有4个样品经PCR测定为内格勒氏菌属阳性,其中只有两个样品显示了福勒猪笼草的阳性扩增。禽N.fowleri菌株的序列分析属于基因型II。