Regulatory small RNAs (sRNAs) ubiquitously impact bacterial physiology through antisense-mediated control of mRNA translation and stability. In Gram negative bacteria, sRNAs often associate with RNA-binding proteins (RBPs), both to gain cellular stability and to enable regulatory efficiency. The Hfq and CsrA proteins were for long the only known global RBPs implicated in sRNA biology. During the last five years, the FinO domain-containing protein ProQ has emerged as another global RBP with a broad spectrum of sRNA and mRNA ligands. This review provides a summary of the current knowledge of enterobacterial ProQ, with a special focus on RNA binding activity, RNA ligand preferences, influence on RNA stability and gene expression, and impact on bacterial physiology. Considering that characterization of ProQ is still in its infancy, we highlight aspects that, when addressed, will provide important clues to the physiological functions and regulatory mechanisms of this globally acting RBP.

调节性小RNA（sRNA）通过反义介导的mRNA翻译和稳定性控制普遍影响细菌生理。在革兰氏阴性细菌中，sRNA经常与RNA结合蛋白（RBP）结合，既能获得细胞稳定性，又能提高调节效率。长期以来，Hfq和CsrA蛋白是唯一涉及sRNA生物学的已知全球RBP。在过去的五年中，含FinO域的蛋白质ProQ成为另一种具有广泛sRNA和mRNA配体的全球RBP。这篇综述总结了目前肠杆菌ProQ的知识，特别关注RNA结合活性，RNA配体偏好，对RNA稳定性和基因表达的影响以及对细菌生理的影响。考虑到ProQ的表征仍处于起步阶段，