Plant Molecular Biology ( IF 5.1 ) Pub Date : 2020-06-19 , DOI: 10.1007/s11103-020-01019-6 Sophie M Brouwer 1 , Firuz Odilbekov 1 , Dharani Dhar Burra 1 , Marit Lenman 1 , Pete E Hedley 2 , Laura Grenville-Briggs 1 , Erik Alexandersson 1 , Erland Liljeroth 1 , Erik Andreasson 1
Using disease bioassays and transcriptomic analysis we show that intact SA-signalling is required for potato defences against the necrotrophic fungal pathogen Alternaria solani.
AbstractSection AbstractEarly blight, caused by the necrotrophic fungus Alternaria solani, is an increasing problem in potato cultivation. Studies of the molecular components defining defence responses to A. solani in potato are limited. Here, we investigate plant defence signalling with a focus on salicylic acid (SA) and jasmonic acid (JA) pathways in response to A. solani. Our bioassays revealed that SA is necessary to restrict pathogen growth and early blight symptom development in both potato foliage and tubers. This result is in contrast to the documented minimal role of SA in resistance of Arabidopsis thaliana against necrotrophic pathogens. We also present transcriptomic analysis with 36 arrays of A. solani inoculated SA-deficient, JA-insensitive, and wild type plant lines. A greater number of genes are differentially expressed in the SA-deficient mutant plant line compared to the wild type and JA- insensitive line. In wild type plants, genes encoding metal ion transporters, such as copper, iron and zinc transporters were upregulated and transferase-encoding genes, for example UDP-glucoronosyltransferase and Serine-glyoxylate transferase, were downregulated. The SA-deficient plants show upregulation of genes enriched in GO terms related to oxidoreductase activity, respiratory chain and other mitochondrial-related processes. Pathogenesis-related genes, such as genes encoding chitinases and PR1, are upregulated in both the SA-deficient and wild type plants, but not in the JA-insensitive mutants. The combination of our bioassays and the transcriptomic analysis indicate that intact SA signalling, and not JA signalling, is required for potato defences against the necrotrophic pathogen A. solani.
中文翻译:
完整的水杨酸信号是马铃薯防御坏死性真菌链格孢菌所必需的。
AbstractSection关键消息
使用疾病的生物分析和转录组学分析,我们表明完整的SA信号传递是马铃薯防御坏死性真菌病原体链格孢菌(Alternaria solani)所必需的。
摘要部分摘要坏死性真菌solternaria solani引起的早疫病是马铃薯栽培中日益严重的问题。限定马铃薯中对茄sol曲霉防御反应的分子成分的研究是有限的。在这里,我们调查植物防御信号,重点关注水杨酸(SA)和茉莉酸(JA)途径对A. solani的响应。我们的生物测定表明,SA对于限制马铃薯叶和块茎中病原体的生长和早期疫病症状的发展是必要的。该结果与文献记载的SA在拟南芥对坏死性病原体的抗性中的最小作用相反。随着36列,我们也存在转录组分析A.菌接种SA缺陷型,JA不敏感型和野生型植物株。与野生型和JA不敏感的品系相比,在SA缺陷型突变植物品系中差异表达大量基因。在野生型植物中,编码金属离子转运蛋白(例如铜,铁和锌转运蛋白)的基因被上调,而编码转移酶的基因(例如UDP-葡萄糖基糖基转移酶和丝氨酸-乙醛酸转移酶)被下调。缺乏SA的植物显示富含GO的基因上调,这些基因与氧化还原酶活性,呼吸链和其他线粒体相关过程有关。发病相关在缺乏SA的植物和野生型植物中,这些基因(例如编码几丁质酶和PR1的基因)均被上调,但对JA不敏感的突变体中却没有上调。我们的生物测定法和转录组分析相结合,表明完整的SA信号而不是JA信号是马铃薯防御坏死性病原体A. solani所必需的。