Palms are important components of tropical and subtropical ecosystems and have even been considered keystone plant resources that can support a large array of pollinators and frugivores. Palms are also economically important. Chamaedorea tepejilote Liebm. is a widely distributed palm with important bioeconomic potential for food, traditional medicine and ornamental purposes. Eighteen microsatellite primers were developed for C. tepejilote. Polymorphism and genetic diversity were evaluated in 71 individuals from four populations in Costa Rica. Thirteen loci were polymorphic and the number of alleles in the pooled sample ranged between 5 and 20, the average number of alleles was 10.61. Average observed heterozygosity was Ho = 0.607 ± 0.04 (SD) and the average expected heterozygosity was He = 0.600 ± 0.03. The exclusion probability of the combined 13 loci, was P_E = 0.998. We tested transferability of the markers in the congeneric C. costaricana, C. pinnantifrons and C. macrospadix. Dioecious species are common in tropical forests; however, few studies have analyzed gene flow patterns in these species. The markers developed for C. tepejilote are an important tool to quantify gene flow patterns and the distribution of genetic diversity within populations. This information will be useful for the development of conservation and management practices of this dioecious tropical palm species.

棕榈树是热带和亚热带生态系统的重要组成部分，甚至被认为是可以支持大量传粉者和食果动物的重要植物资源。棕榈树在经济上也很重要。Chamaedorea tepejilote Liebm。是一种分布广泛的棕榈树，具有重要的食品、传统医药和观赏用途的生物经济潜力。为C. tepejilote开发了 18 个微卫星引物. 对来自哥斯达黎加四个种群的 71 名个体进行了多态性和遗传多样性评估。13 个位点具有多态性，合并样本中等位基因的数量介于 5 到 20 之间，等位基因的平均数量为 10.61。观察到的平均杂合性为 Ho = 0.607 ± 0.04 (SD)，平均预期杂合性为 He = 0.600 ± 0.03。组合的 13 个位点的排除概率为 P _E  = 0.998。我们测试了同类C. costaricana、C. pinnantifrons和C. macrospadix中标记的可转移性。雌雄异株的物种在热带森林中很常见；然而，很少有研究分析这些物种的基因流动模式。为C. tepejilote开发的标记是量化基因流动模式和种群内遗传多样性分布的重要工具。这些信息将有助于开发这种雌雄异株的热带棕榈树种的保护和管理实践。