Natural compounds extracted from marine organisms consisting of biological active materials like collagen provide a major source of compound with medical applications including wound dressing particularly in co-treatment with other natural or synthetic materials to quick the wound healing process. The current study investigates the potential of Rutilus kutum skin-extracted collagen in combination with chitosan [collagen-chitosan (Col-CH) gel] for in vivo second-degree burn wound healing in rats following finding of the most effective collagen-chitosan ratio. Collagen and chitosan were extracted from R. Kutum skin and shell waste of shrimp, respectively, using acid–base method. SDS-PAGE was performed to determine subunit composition of collagen. After amino acid analysis using HPLC, different ratios of collagen and chitosan mixture (Col-CH, 1:3, 1:1 and 3:1) were prepared to fine the most effective of Col-CH gel on burn wound healing and compare to animals treated with silver sulfadiazine ointment. Wound surface area was analyzed using ImageJ software on days 15 and 30. H&E staining was performed to show the epithelialization, collagen deposition, fibroblast cell accumulation and vessel formation on days 15 and 30. The results from H&E were scored and quantified by pathologist. SDS-PAGE technique revealed that extracted-collagen was type 1 composed of two α (α₁ and α₂) chains. HPLC showed that Glycine was the most abundant amino acid which included one-third of total amino acid in the skin. The wound surface measurement showed a significant reduction in wound size in animals treated with Col-CH (1:1) compared to silver sulfadiazine treated group at day 25. Histopathological findings represented high score in epithelialization, vessel formation and a decrease in inflammatory cells infiltration in Col-CH (1:1) treated group on days 15 and 30. These data suggest collagen in combination with chitosan with 1:1 ratio can significantly restore burn wound compared to silver sulfadiazine ointment.

从海洋生物中提取的由生物活性物质（如胶原蛋白）组成的天然化合物为医疗应用提供了主要的化合物来源，包括伤口敷料，特别是在与其他天然或合成材料共同治疗以加快伤口愈合过程的过程中。当前的研究调查了发现最有效的胶原蛋白-壳聚糖比率后，R麻皮肤提取的胶原蛋白与壳聚糖[胶原-壳聚糖（Col-CH）凝胶]联合在大鼠体内二级烧伤创面愈合中的潜力。胶原蛋白和壳聚糖从R.Kutum提取虾皮和壳废料分别采用酸碱法处理。进行SDS-PAGE以确定胶原的亚基组成。使用HPLC进行氨基酸分析后，制备了不同比例的胶原蛋白和壳聚糖混合物（Col-CH，1：3、1：1和3：1），以筛选最有效的Col-CH凝胶对烧伤创面愈合，并与用磺胺嘧啶银软膏治疗的动物。在第15天和第30天使用ImageJ软件分析伤口的表面积。在第15天和第30天进行H＆E染色以显示上皮化，胶原蛋白沉积，成纤维细胞积累和血管形成。H＆E的结果由病理学家评分和定量。SDS-PAGE技术透露，提取胶原是1型2个α（组成α ₁和α ₂）连锁店。HPLC显示，甘氨酸是最丰富的氨基酸，占皮肤总氨基酸的三分之一。伤口表面测量显示，与第25天的磺胺嘧啶银治疗组相比，用Col-CH（1：1）治疗的动物的伤口大小明显减少。组织病理学结果表明上皮形成，血管形成和炎症细胞浸润减少得分高在第15天和第30天接受Col-CH（1：1）治疗组的实验结果。这些数据表明，与磺胺嘧啶银软膏相比，胶原蛋白与脱乙酰壳多糖的比例为1：1的胶原蛋白可以显着恢复烧伤创面。