We developed single nucleotide polymorphism (SNP) markers to support a genetics-based capture-mark-recapture (CMR) project implemented for the management of Pacific walrus (Odobenus rosmarus divergens). Using a combination of Restriction-site Associated DNA sequencing (RADSeq) and genome resequencing, 57,504 single nucleotide variants were identified from Pacific walrus DNA. RADSeq was performed on genomic DNA from 192 walruses (63 males; 94 females; 35 unknown sex) whereas genome resequencing was performed on 41 individual walruses (21 males, 20 females). All read mappings were subjected to quality-based variant detection and screened for SNPs. These efforts generated a pool of 909 putative SNPs from which 217 unique TaqMan® assays were developed for validation. Ultimately, 116 validated, biallelic SNPs were combined to create a genetic tag for identifying individual walrus during the multi-year CMR project. Conversion to a 116-SNP TaqMan® OpenArray® enabled use of a high-throughput genotyping qPCR platform, which expedited accurate discrimination of individual walrus and reduced the probability of genotyping errors, thereby providing improved resolution for assessing the size and demographic rates of this population.

我们开发了单核苷酸多态性（SNP）标记，以支持基于遗传学的捕获标记捕获（CMR）项目，该项目实施用于管理太平洋海象（Odobenus rosmarus divergens）。结合限制性位点相关DNA测序（RADSeq）和基因组重测序，从太平洋海象DNA中鉴定出57,504个单核苷酸变体。对来自192只海象（63名男性； 94名女性； 35名未知性别）的基因组DNA进行RADSeq，而对41只海象（21名男性，20名女性）进行基因组重测序。所有读取的映射均经过基于质量的变异检测，并筛选出SNP。这些努力产生了909个推定的SNP，从中开发了217种独特的TaqMan®分析进行验证。最终，在多年的CMR项目中，结合了116个经过验证的双等位基因SNP，创建了一个用于识别单个海象的遗传标签。转换为116-SNPTaqMan®OpenArray®可以使用高通量基因分型qPCR平台，