β-glucosidases have received considerable attention due to their essential role in bioethanol production from lignocellulosic biomass. β-glucosidase can hydrolyse cellobiose in cellulose degradation and its low activity has been considered as one of the main limiting steps in the process. Large-scale conversions of cellulose therefore require high enzyme concentration which increases the cost. β-glucosidases with improved activity and thermostability are therefore of great commercial interest. The fungus Trichoderma reseei expresses thermostable cellulolytic enzymes which have been widely studied as attractive targets for industrial applications. Genetically modified β-glucosidases from Trichoderma reseei have been recently commercialised. We have developed an approach in which screening of low molecular weight molecules (fragments) identifies compounds that increase enzyme activity and are currently characterizing fragment-based activators of TrBgl2. A structural analysis of the 55 kDa apo form of TrBgl2 revealed a classical (α/β)₈-TIM barrel fold. In the present study we present a partial assignment of backbone chemical shifts, along with those of the Ile (I)-Val (V)-Leu (L) methyl groups of TrBgl2. These data will be used to characterize the interaction of TrBgl2 with the small molecule activators.

中文翻译：

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来自里氏木霉的真菌 β-葡萄糖苷酶的 1H、13C、15N 骨架和 IVL 甲基基团共振分配。

β-葡萄糖苷酶因其在木质纤维素生物质生物乙醇生产中的重要作用而受到广泛关注。β-葡萄糖苷酶可以水解纤维素降解中的纤维二糖，其低活性被认为是该过程中的主要限制步骤之一。因此，纤维素的大规模转化需要高酶浓度，这会增加成本。因此，具有改善的活性和热稳定性的 β-葡萄糖苷酶具有很大的商业价值。真菌Trichoderma reseei表达热稳定的纤维素分解酶，这些酶已被广泛研究为工业应用的有吸引力的目标。来自Trichoderma reseei 的转基因 β-葡萄糖苷酶最近已经商业化。我们开发了一种方法，其中低分子量分子（片段）的筛选可识别增加酶活性的化合物，目前正在表征基于片段的Tr Bgl2激活剂。Tr Bgl2的 55 kDa apo 形式的结构分析揭示了经典的 (α/β) ₈ -TIM 桶状折叠。在本研究中，我们提出了主链化学位移的部分分配，以及Tr Bgl2的 Ile (I)-Val (V)-Leu (L) 甲基基团的化学位移。这些数据将用于表征Tr Bgl2 与小分子活化剂的相互作用。