Formin-like genes (FMNLs) are members of formins family and have been implicated to the development and progression of multiple cancers. This research aims to analyze the expression profiles, prognostic values, and immune infiltrating associations of FMNLs in gastric cancer (GC) using multiple online bioinformatics website, including Oncomine, UALCAN, Kaplan–Meier Plotter, TIMER, GeneMANIA, DAVID, and LinkedOmics databases. The mRNA levels of FMNL1/2/3 were higher in GC tissues than normal. Meanwhile, FMNLs expressions tend to be upregulated in advanced and poorly differentiated GC. Prognostic value analysis suggested that high transcription levels of FMNL1/3 were associated with poor overall survival in GC patients. Correlation analysis between FMNLs expressions and immune infiltrating GC revealed that the expressions of FMNLs were significantly associated with immune infiltrating. Protein–protein interaction network and enrichment analysis of FMNLs in GC showed that FMNLs coexpressed genes mainly participated in organizing actin cytoskeleton through affecting small G proteins activity. Moreover, Gene Set Enrichment Analysis (GSEA) analysis uncovered FMNLs and their coexpressed genes was tightly associated with immune-related cellular functions. These findings demonstrate that FMNLs might play significant immunomodulatory roles in tumor immunity and could be novel therapeutic targets and potential prognostic biomarkers in GC.

中文翻译：

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Formin样基因家族在胃癌中的表达和预后价值的系统表征。

Formin样基因（FMNL）是formins家族的成员，与多种癌症的发生和发展有关。这项研究旨在使用多个在线生物信息学网站，包括Oncomine，UALCAN，Kaplan-Meier Plotter，TIMER，GeneMANIA，DAVID和LinkedOmics数据库，分析FMNL在胃癌（GC）中的表达特征，预后价值和免疫浸润关联。GC组织中FMNL1 / 2/3的mRNA水平高于正常组织。同时，FMNLs表达倾向于在晚期和低分化的GC中上调。预后价值分析表明，FMNL1 / 3的高转录水平与GC患者的整体生存期较差有关。FMNLs表达与免疫浸润GC之间的相关性分析表明，FMNLs的表达与免疫浸润显着相关。蛋白质-蛋白质相互作用网络和FMNLs在GC中的富集分析表明，FMNLs共表达的基因主要通过影响小G蛋白活性而参与组织肌动蛋白细胞骨架。此外，基因集富集分析（GSEA）分析发现了FMNL及其共表达的基因与免疫相关的细胞功能紧密相关。这些发现表明，FMNLs可能在肿瘤免疫中发挥重要的免疫调节作用，并且可能是GC中的新型治疗靶点和潜在的预后生物标志物。蛋白质-蛋白质相互作用网络和FMNLs在GC中的富集分析表明，FMNLs共表达的基因主要通过影响小G蛋白活性而参与组织肌动蛋白细胞骨架。此外，基因集富集分析（GSEA）分析发现了FMNL及其共表达的基因与免疫相关的细胞功能紧密相关。这些发现表明，FMNLs可能在肿瘤免疫中发挥重要的免疫调节作用，并且可能是GC中的新型治疗靶点和潜在的预后生物标志物。蛋白质-蛋白质相互作用网络和FMNLs在GC中的富集分析表明，FMNLs共表达的基因主要通过影响小G蛋白活性而参与组织肌动蛋白细胞骨架。此外，基因集富集分析（GSEA）分析发现了FMNL及其共表达的基因与免疫相关的细胞功能紧密相关。这些发现表明，FMNLs可能在肿瘤免疫中发挥重要的免疫调节作用，并且可能是GC中的新型治疗靶点和潜在的预后生物标志物。