Molecular computing offers a powerful framework for in situ biosensing and signal processing at the nanoscale. However, for in vivo applications, the use of conventional DNA components can lead to false positive signals being generated due to degradation of circuit components by nuclease enzymes. Here, we use hybrid chiral molecules, consisting of both l- and d-nucleic acid domains, to implement leakless signal translators that enable d-nucleic acid signals to be detected by hybridization and then translated into a robust l-DNA signal for further analysis. We show that our system is robust to false positive signals even if the d-DNA components are degraded by nucleases, thanks to circuit-level robustness. This work thus broadens the scope and applicability of DNA-based molecular computers for practical, in vivo applications.

中文翻译：

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使用无泄漏平移器进行稳固的异位股线位移。

分子计算为纳米原位生物传感和信号处理提供了强大的框架。然而，对于体内应用，由于核酸酶降解电路组分，常规DNA组分的使用会导致产生假阳性信号。在这里，我们使用由l-和d-核酸域组成的杂合手性分子来实现无泄漏信号转换器，该信号转换器使d-核酸信号能够通过杂交检测，然后转化为可靠的l -DNA信号以进行进一步分析。我们证明，即使d-DNA成分由于电路水平的坚固性而被核酸酶降解。因此，这项工作拓宽了基于DNA的分子计算机在体内实际应用中的范围和适用性。