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Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing.
Emerging Microbes & Infections ( IF 13.2 ) Pub Date : 2020-06-18 , DOI: 10.1080/22221751.2020.1775500 Fabiola Mancini 1 , Fabrizio Barbanti 1 , Maria Scaturro 1 , Giulia Errico 1, 2 , Angelo Iacobino 1 , Antonino Bella 1 , Flavia Riccardo 1 , Giulia Marsili 1 , Paola Stefanelli 1 , Patrizio Pezzotti 1 , Giovanni Rezza 1 , Alessandra Ciervo , 1
中文翻译：
SARS-CoV-2检测的实验室管理：热处理方法和rt-实时PCR测试的用户友好组合。
更新日期：2020-06-18
Emerging Microbes & Infections ( IF 13.2 ) Pub Date : 2020-06-18 , DOI: 10.1080/22221751.2020.1775500 Fabiola Mancini 1 , Fabrizio Barbanti 1 , Maria Scaturro 1 , Giulia Errico 1, 2 , Angelo Iacobino 1 , Antonino Bella 1 , Flavia Riccardo 1 , Giulia Marsili 1 , Paola Stefanelli 1 , Patrizio Pezzotti 1 , Giovanni Rezza 1 , Alessandra Ciervo , 1
Affiliation
ABSTRACT
The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.
中文翻译：
SARS-CoV-2检测的实验室管理：热处理方法和rt-实时PCR测试的用户友好组合。
摘要
RNA纯化是检测拭子样品中SARS-CoV-2的金标准，但这取决于化学试剂的可用性。在这项研究中，我们评估了不提取RNA的热处理方法作为核酸纯化的可靠选择。
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