The fruit fly Drosophila melanogaster is a model species used for a wide range of studies. Contamination of Drosophila cultures with bacterial infection is common and is readily eradicated by antibiotics. Neomycin antibiotics can cause stress to D. melanogaster’s larvae and imagoes, which may affect the interpretation of the results of research using culture from neomycin-based medium. In the present study, fluctuating asymmetry (FA), one of the important bioindicators of stress, was measured. Larvae and imagoes of a wild-type D. melanogaster strain were exposed to various concentrations of neomycin. The size of anal papillae and selected wing veins were measured using scanning electron and light microscopy, respectively. Next, the FA was checked. The values obtained for larval anal papillae appeared to be concentration-dependant; the FA indices increased with the concentration of neomycin. The wing FA presented a large but variable correlation, depending on the measured vein. However, the mean length of veins was the highest for the control group, with neomycin-exposed groups showing lower values. The research showed that neomycin may cause sublethal stress in D. melanogaster, which manifests in increased FA indices. This suggests that neomycin can cause physiological and developmental stress in insects, which should be taken into account when interpreting the results of studies using these model organisms.

中文翻译：

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果蝇对以新霉素为基础的培养基以波动不对称表示的饲喂反应。

果蝇果蝇是用于范围广泛的研究模型物种。果蝇培养物受细菌感染的污染很普遍，很容易被抗生素根除。新霉素抗生素会给黑腹果蝇的幼虫和成虫造成压力，这可能会影响使用基于新霉素的培养基进行培养的研究结果的解释。在本研究中，测量了波动的不对称性（FA），它是压力的重要生物指标之一。野生型D. melanogaster的幼虫和成虫菌株暴露于各种浓度的新霉素。使用扫描电子显微镜和光学显微镜分别测量肛门乳头和选定的翼状静脉的大小。接下来，检查FA。幼虫肛门乳头获得的值似乎是浓度依赖性的。FA指数随新霉素浓度的增加而增加。机翼FA呈现出很大但可变的相关性，具体取决于所测量的静脉。但是，对照组的平均静脉长度最高，新霉素暴露组的平均血管长度较低。研究表明新霉素可能会导致黑腹果蝇的致死性压力，这表现为FA指数增加。这表明新霉素可引起昆虫的生理和发育应激，在解释使用这些模型生物的研究结果时应考虑到这一点。