A biopesticide that combines Metarhizium rileyi and nucleopolyhedrovirus (NPV) in an oil dispersion (OD) was developed. Its efficacy against Anticarsia gemmatalis and Chrysodeixis includens was evaluated under laboratory and field conditions. First, each of the two selected fungal strains, combined or not in OD preparations with AgMNPV or ChinNPV, was tested in the laboratory against A. gemmatalis and C. includens, respectively. AgMNPV–fungus combinations had similar effect on overall mortality of A. gemmatalis compared to each pathogen used alone. C. includens was less susceptible to infection by M. rileyi strains, and ChinNPV became the major cause of larval death. The OD formulations with M. rileyi and/or NPV were applied in infested soybean fields. ChinNPV + M. rileyi caused 50.8% of overall mortality six days after spraying in a soybean crop infested by both pests, whereas for treatments with the fungus or the virus alone the percentages were 41.2% and 8.8%, respectively. In another field infested solely by A. gemmatalis, insect mortality was similar for the AgMNPV–fungus combination and the virus used alone. Larval parasitism by wasps and dipterans was not affected following biopesticide spraying. Our results suggest that the performance of a dual‐action biopesticide is related to the NPV and fungal strains that are combined and the proportion of host populations simultaneously infesting the crop.

中文翻译：

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利里氏根瘤菌和核多角体病毒联合油基制剂对大豆鳞翅目害虫的功效

开发了一种在油分散液（OD）中结合了利落弧菌和核多角体病毒（NPV）的生物农药。在实验室和野外条件下评估了其对双生抗Carcarmatalis和Chrysodeixis includens的功效。首先，每个所选择的两个真菌菌株，结合或不OD制剂与AgMNPV或ChinNPV，在针对实验室测试A.藜豆夜蛾和C. includens分别。与单独使用每种病原体相比，AgMNPV-真菌组合对双歧杆菌的总死亡率具有相似的影响。C.includens对M. rileyi的感染较不敏感菌株，而ChinNPV成为幼虫死亡的主要原因。具有里氏木霉和/或NPV的OD制剂被用于受侵染的大豆田。在被两种病虫侵染的大豆作物上喷洒六天后，ChinNPV +  rileyi导致总死亡率的50.8％，而仅用真菌或病毒处理的百分率分别为41.2％和8.8％。在另一个仅由双歧杆菌感染的领域，AgMNPV-真菌组合和单独使用病毒的昆虫死亡率相似。喷洒生物农药后，黄蜂和dip虫的幼虫寄生虫不会受到影响。我们的结果表明，双重作用生物农药的性能与NPV和真菌菌株的结合以及宿主种群同时侵染作物的比例有关。