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Hyperspectral characterization of re-epithelialization in an in vitro wound model.
Journal of Biophotonics ( IF 2.8 ) Pub Date : 2020-06-17 , DOI: 10.1002/jbio.202000108
Asgeir Bjorgan 1 , Brita S Pukstad 2, 3 , Lise L Randeberg 1
Affiliation  

In vitro wound models are useful for research on wound re‐epithelialization. Hyperspectral imaging represents a non‐destructive alternative to histology analysis for detection of re‐epithelialization. This study aims to characterize the main optical behavior of a wound model in order to enable development of detection algorithms. K‐Means clustering and agglomerative analysis were used to group spatial regions based on the spectral behavior, and an inverse photon transport model was used to explain differences in optical properties. Six samples of the wound model were prepared from human tissue and followed over 22 days. Re‐epithelialization occurred at a mean rate of 0.24 mm2/day after day 8 to 10. Suppression of wound spectral features was the main feature characterizing re‐epithelialized and intact tissue. Modeling the photon transport through a diffuse layer placed on top of wound tissue properties reproduced the spectral behavior. The missing top layer represented by wounds is thus optically detectable using hyperspectral imaging.image

中文翻译:

在体外伤口模型中重新上皮化的高光谱表征。

体外伤口模型对于伤口再上皮形成的研究很有用。高光谱成像代表了组织学分析的非破坏性替代方法,用于检测上皮细胞的再上皮形成。这项研究旨在表征伤口模型的主要光学行为,以便开发检测算法。使用K均值聚类和凝聚分析根据光谱行为对空间区域进行分组,并使用反光子传输模型来解释光学性质的差异。从人体组织中制备了六个伤口模型样品,然后进行了22天。上皮再形成的平均发生率为0.24 mm 2从第8天到第10天的第2天/天,伤口频谱特征的抑制是表征上皮化和完整组织的主要特征。通过放置在伤口组织属性顶部的扩散层对光子传输进行建模,可以再现光谱行为。因此,使用高光谱成像可以光学检测到伤口所代表的缺失顶层。图片
更新日期:2020-06-17
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