This study investigated the impacts of selective sole carbon source-induced micropollutants (MPs) cometabolism of Chlorella sp. by: (i) extracellular polymeric substances (EPS), superoxide dismutase and peroxidase enzyme production; (ii) MPs removal efficiency and cometabolism rate; (iii) MPs’ potential degradation products identification; and (iv) degradation pathways and validation using the Eawag database to differentiate the cometabolism of Chlorella sp. with other microbes. Adding the sole carbon sources in the presence of MPs increased EPS and enzyme concentrations from 2 to 100-fold in comparison with only sole carbon sources. This confirmed that MPs cometabolism had occurred. The removal efficiencies of tetracycline, sulfamethoxazole, and bisphenol A ranged from 16-99%, 32–92%, and 58–99%, respectively. By increasing EPS and enzyme activity, the MPs concentrations accumulated in microalgae cells also fell 400-fold. The cometabolism process resulted in several degradation products of MPs. This study drew an insightful understanding of cometabolism for MPs remediation in wastewater. Based on the results, proper carbon sources for microalgae can be selected for practical applications to remediate MPs in wastewater while simultaneously recovering biomass for several industries and gaining revenue.

这项研究调查了小球藻的选择性唯一碳源诱导的微污染物（MPs）代谢的影响。通过：（i）细胞外聚合物（EPS），超氧化物歧化酶和过氧化物酶的产生；（ii）MP清除效率和新陈代谢率；（iii）国会议员的潜在降解产物识别；（iv）降解途径和使用Eawag数据库进行验证以区分小球藻的新陈代谢sp。与其他微生物。与仅有的碳源相比，在MPs存在下添加唯一的碳源可使EPS和酶浓度增加2到100倍。这证实了MP发生了新陈代谢。四环素，磺胺甲恶唑和双酚A的去除效率分别为16-99％，32-92％和58-99％。通过增加EPS和酶活性，微藻细胞中积累的MP浓度也下降了400倍。代谢过程导致MP的几种降解产物。这项研究对废水中MP修复的新陈代谢有了深刻的了解。根据结果​​，