The role of the cotyledonary haustorium (CH) in the mobilization of nutrient reserves in the endosperm of species of the palm family Arecaceae is a moot question. To shed light on this matter, we present here an analysis of the quantitative proteome changes associated with four developmental stages of CH and three of endosperm during germination. Together, a total of 1965 proteins were identified, being 1538 in the CH and 960 in the endosperm. Both in the CH and endosperm proteomes, we observed an increase in the diversity of hydrolases as the CH and endosperm develops. Qualitative proteomics analysis of four CH developmental stages indicated that each stage is populated by a unique set of proteins and the quantitative analysis showed an increase in the relative abundance of hydrolases, particularly mannan degrading enzymes, as development progresses. These results add weight to the hypothesis that the CH in the seeds of E. oleraceaacts both as a conduit of carbon and nitrogen sources generated by the hydrolysis of the reserves in the endosperm and as a source of hydrolases that will contribute to the mobilization of these reserves.

中文翻译：

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Euterpe oleracea种子萌发过程中子叶Haustorium和胚乳的蛋白质组动力学

子叶吸器 (CH) 在棕榈科槟榔科物种胚乳中营养储备的动员中的作用是一个有争议的问题。为了阐明这个问题，我们在此分析了与发芽过程中 CH 的四个发育阶段和胚乳的三个发育阶段相关的定量蛋白质组变化。总共鉴定了 1965 种蛋白质，其中 1538 种在 CH 中，960 种在胚乳中。在 CH 和胚乳蛋白质组中，我们观察到随着 CH 和胚乳的发育，水解酶的多样性增加。四个 CH 发育阶段的定性蛋白质组学分析表明，每个阶段都由一组独特的蛋白质组成，定量分析显示水解酶的相对丰度增加，特别是甘露聚糖降解酶，随着开发的进展。这些结果增加了以下假设的重要性：E. oleracea 种子中的 CH 既是胚乳中储备物水解产生的碳源和氮源的管道，又是有助于动员这些碳源和氮源的水解酶源。储备。