The presence of organophosphate esters (OPEs) in everyday commodities such as furniture, household appliances and baby toys have rendered these contaminants ubiquitous in environmental fates such as air, water, soils and biota. Their presence in food-related species suggests that an additional route of exposure to these esters for the general population is fish intake through diet. Their incipient toxicity and carcinogenetic behaviour make it essential to develop methods for determining OPEs in fish samples. In this paper we have developed a new method for determining 9 OPEs based on the QuEChERS extraction method followed by a simple clean-up using a novel device for selective lipid removal (LipiFiltr) and GC–MS/MS to extract these compounds from fish samples regardless of lipid content. QuEChERS salt packet optimisation and clean-up strategies such as liquid-liquid extraction, dispersive-solid phase extraction and LipiFiltr were tested. Our results showed that EN 15662 method salts and Lipifiltr were the best combination to produce efficient analyte apparent recovery (67–116%) and negligible matrix effects (<10%). Limits of detection ranged from 0.05 ng g⁻¹ (dry weight) for TiBP and TBP to 2.00 ng g⁻¹ (dry weight) for TCEP. Fish samples from four fish species were determined with a median concentration of ΣOPEs 5.31 ng g⁻¹ on a wet weight basis, with TBP, TiBP and TCPP as the main contenders. Estimates of exposure and risk associated with consuming these compounds via dietary intake showed low levels of concern for the population of Tarragona.

在家具，家用电器和婴儿玩具等日常用品中存在有机磷酸酯（OPE），这些污染物在空气，水，土壤和生物群等环境命运中普遍存在。它们在与食物有关的物种中的存在表明，普通人群接触这些酯的另一种途径是通过饮食摄入鱼。它们的初期毒性和致癌行为使得开发测定鱼样品中OPE的方法至关重要。在本文中，我们开发了一种基于QuEChERS提取方法测定9种OPE的新方法，然后使用新型脂质选择性去除装置（LipiFiltr）和GC-MS / MS进行简单清理，以从鱼类样品中提取这些化合物无论脂质含量如何。测试了QuEChERS盐包的优化和净化策略，例如液-液萃取，分散固相萃取和LipiFiltr。我们的结果表明，EN 15662方法的盐和Lipifiltr是产生有效分析物表观回收率（67–116％）和可忽略的基质效应（<10％）的最佳组合。检出限为0.05 ng g^-1（干重）为TIBP和TBP〜2.00纳克克^-1（干重）为TCEP。确定了四种鱼类的鱼样品，以湿重计，其中位数浓度为ΣOPEs5.31 ng g ^-1，其中主要成分为TBP，TiBP和TCPP。通过饮食摄入与摄入这些化合物有关的接触和风险的估计表明，塔拉戈纳（Tarragona）人群的关注水平较低。