Abstract In recent years, the incidence of apple canker disease is increasing and assuming an epidemic in major apple growing parts of Jammu and Kashmir. During 2017–18, canker disease incidence was observed in all the apple growing regions surveyed, with characteristic symptoms of smoky canker. Total 40 samples were collected from regions of north, central and south Kashmir from three different varieties, Red Delicious, Royal Delicious and Golden Delicious. Among 40 samples collected total 20 isolates representing all the three varieties and regions were maintained for morpho-molecular characterization. Based on cultural and morphological characteristics, the fungus was identified as genus Diplodia with two morphological groups. Sequence based analysis of the internal transcribed spacer region (ITS) and elongation factor (EF1) gene confirmed the species as Diplodia bulgarica and Diplodia seriata. The Diplodia bulgarica was found predominant species in all the three regions surveyed. The phylogenetic analysis of both the genes clustered the two species in two different groups. The pathogenicity was confirmed on two-year-old potted plants of apple cv. Red Delicious. This study would help in better understanding the role of Diplodia species on symptom development, epidemiology and formulation of improved disease management strategies against apple canker.

中文翻译：

---

Diplodia spp 的形态和分子特征。与印度的苹果溃疡病有关

摘要 近年来，苹果溃疡病的发病率呈上升趋势，并在查谟和克什米尔的主要苹果产区呈流行趋势。2017-18 年期间，在所有调查的苹果种植区都观察到溃疡病发病率，具有烟状溃疡病的特征性症状。从克什米尔北部、中部和南部地区收集了 40 个样品，分别来自红冠、皇家美味和金冠三个不同品种。在收集的 40 个样品中，共有 20 个代表所有三个品种和地区的分离株被保留用于形态分子表征。根据培养和形态特征，该真菌被鉴定为具有两个形态组的双倍体属。内部转录间隔区 (ITS) 和延伸因子 (EF1) 基因的基于序列的分析证实该物种为 Diplodia bulgarica 和 Diplodia seriata。Diplodia bulgarica 是所有三个调查地区的主要物种。这两个基因的系统发育分析将这两个物种分为两个不同的组。致病性在苹果品种的两年生盆栽植物上得到证实。红色美味。这项研究将有助于更好地了解双倍体物种在症状发展、流行病学和制定针对苹果溃疡病的改进疾病管理策略方面的作用。这两个基因的系统发育分析将这两个物种分为两个不同的组。致病性在苹果品种的两年生盆栽植物上得到证实。红色美味。这项研究将有助于更好地了解双倍体物种在症状发展、流行病学和制定针对苹果溃疡病的改进疾病管理策略方面的作用。这两个基因的系统发育分析将这两个物种分为两个不同的组。致病性在苹果品种的两年生盆栽植物上得到证实。红色美味。这项研究将有助于更好地了解双倍体物种在症状发展、流行病学和制定针对苹果溃疡病的改进疾病管理策略方面的作用。