Algal Research ( IF 5.1 ) Pub Date : 2020-06-17 , DOI: 10.1016/j.algal.2020.101970 Stefanie Böhmer , Christina Marx , Álvaro Gómez-Baraibar , Marc M. Nowaczyk , Dirk Tischler , Anja Hemschemeier , Thomas Happe
Old Yellow Enzymes (OYEs) selectively reduce carbon‑carbon double bonds of a broad range of substrates with excellent stereoselectivity. Current challenges of their application in bio-based industrial processes are the cost-efficient regeneration of the nicotinamide co-substrate and expanding the range of industrially relevant substrates. Microalgae represent a rich and mostly untapped source for OYEs with novel catalytic properties and are promising whole-cell factories that regenerate nicotinamide by photosynthesis. By comparative phylogenetics, we identified 20 putative OYEs in eleven algal species. Three recombinant OYEs from the unicellular green alga Chlamydomonas reinhardtii (CrOYEs) reveal diverse biocatalytic properties and potential for enzyme optimization. High substrate conversion activities of living algal wild type and mutant cells linked the biocatalytic profiles to in vivo functionality and demonstrate the potential of microalgae as sources for distinctive OYE activities and for light-driven biotransformations.
中文翻译:
进化多样的莱茵衣藻旧黄色酶显示出独特的催化特性和全细胞生物转化的潜力
旧的黄色酶(OYEs)具有出色的立体选择性,可以选择性还原多种底物的碳-碳双键。它们在生物基工业过程中的应用当前面临的挑战是烟酰胺共基质的经济高效再生以及扩大与工业相关的基质的范围。微藻代表了具有新颖催化特性的OYE的丰富且几乎未开发的来源,并且是有前途的通过光合作用再生烟酰胺的全细胞工厂。通过比较系统发育学,我们在11个藻类物种中鉴定出20个假定的OYE。来自单细胞绿藻莱茵衣藻的三种重组OYE(CrOYEs)具有多种生物催化特性和酶优化潜力。活的藻类野生型和突变细胞的高底物转化活性将生物催化特性与体内功能联系起来,并证明了微藻作为独特的OYE活性和光驱动生物转化来源的潜力。