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Root colonization and arbuscular mycorrhizal fungal community composition in a genetically modified maize, its non-modified isoline, and a landrace.
Mycorrhiza ( IF 3.9 ) Pub Date : 2020-06-15 , DOI: 10.1007/s00572-020-00969-5
Diana Marcela Morales Londoño 1 , Edenilson Meyer 1 , Kelly Justin da Silva 2 , Anabel González Hernández 3 , Rafael Dutra de Armas 2 , Luis Macedo Soares 4 , Sidney Luiz Stürmer 5 , Rubens Onofre Nodari 6 , Cláudio Roberto Fonsêca Sousa Soares 3 , Paulo Emilio Lovato 1
Affiliation  

The use of genetically modified (GM) plants has increased in recent decades, but there are uncertainties about their effects on soil microbial communities. Aiming to quantify root colonization and characterize arbuscular mycorrhizal fungi (AMF) communities associated with roots and rhizosphere soil of different maize genotypes, a field trial was carried out in Southern Brazil with three maize genotypes as follows: a GM hybrid (DKB 240 VTPRO), its non-modified isoline (DKB 240), and a landrace (Pixurum). Soil samples were collected to evaluate the occurrence of AMF during the growth of corn genotypes at sowing and V3 (vegetative), R1 (flowering), and R3 (grain formation) stages of the crop. The occurrence of AMF was determined by the morphological identification of spores, and by analyzing AMF community composition in soil and roots of maize, using PCR-DGGE. The GM genotype of maize promoted lower mycorrhizal colonization in the vegetative stage and had lower sporulation at grain development than the conventional hybrid and the landrace maize. Twenty AMF morphotypes were identified and 13 were associated with all maize genotypes. The genera Acaulospora, Glomus, and Dentiscutata had the largest numbers of species. There were no differences in AMF community composition due to maize genotypes or genetic modification, but crop phenological stages affected AMF communities associated with maize roots.

中文翻译:

转基因玉米、其未修饰的等值线和地方品种中的根定殖和丛枝菌根真菌群落组成。

近几十年来,转基因(GM)植物的使用有所增加,但它们对土壤微生物群落的影响尚不确定。为了量化根系定殖和表征与不同玉米基因型的根和根际土壤相关的丛枝菌根真菌 (AMF) 群落,在巴西南部进行了以下三种玉米基因型的田间试验:转基因杂交种 (DKB 240 VTPRO),其未修饰的等值线 (DKB 240) 和地方品种 (Pixurum)。收集土壤样品以评估玉米基因型在播种和作物的 V3(营养)、R1(开花)和 R3(籽粒形成)阶段生长过程中 AMF 的发生情况。AMF的发生通过孢子的形态鉴定,通过分析玉米土壤和根系的AMF群落组成,使用 PCR-DGGE。与常规杂交玉米和地方品种玉米相比,玉米的转基因基因型在营养阶段促进了较低的菌根定殖,并且在籽粒发育时具有较低的孢子形成。鉴定了 20 种 AMF 形态型,其中 13 种与所有玉米基因型相关。属AcaulosporaGlomusDentiscutata的物种数量最多。由于玉米基因型或基因改造,AMF 群落组成没有差异,但作物物候阶段影响与玉米根相关的 AMF 群落。
更新日期:2020-06-15
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