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Eukaryotic translation initiation factor 3 subunit b is a novel oncogenic factor in prostate cancer.
Mammalian Genome ( IF 2.5 ) Pub Date : 2020-06-15 , DOI: 10.1007/s00335-020-09842-4
Ping Xiang 1, 2 , Youwen Sun 2 , Zhiqing Fang 1 , Keqiang Yan 1 , Yidong Fan 1
Affiliation  

Prostate cancer, the second most common cancer among male adults, affects millions globally. We sought to investigate the expression and contribution of Eukaryotic translation initiation factor 3 subunit b (EIF3B) in prostate cancer. Expression of EIF3B was analyzed in both human prostate patient tissues and prostate cancer cell lines. Small interfering RNA (siRNA) knockdown of EIF3B was introduced into prostate cancer cell line PC-3 and LNCaP, followed by examination of cell viability, proliferation and apoptosis using the MTT, cell counting and terminal deoxynucleotidyl transferase dUTP nick end labeling assays, respectively. An in vivo xenograft tumor mouse model was employed to address the role of EIF3B in tumorigenesis as well. Finally, a gene microarray analysis was performed to search for differentially expressed genes upon EIF3B knockdown. EIF3B was upregulated in prostate tumor tissues and prostate cancer cell lines. EIF3B knockdown inhibited viability and proliferation of prostate cancer cells, as well as promoted cell apoptosis. In the in vivo mouse model, inoculation of EIF3B knockdown PC-3 cells displayed inhibited growth of xenograft tumors. In addition, potential signaling pathways that might be involved in EIF3B action in prostate cancer were identified by the gene microarray. EIF3B is a novel oncogenic factor in prostate cancer both in vitro and in vivo, which could be employed as a novel therapeutic target in the treatment against prostate cancer.



中文翻译:

真核翻译起始因子 3 亚基 b 是一种新型的前列腺癌致癌因子。

前列腺癌是男性成年人中第二常见的癌症,影响着全球数百万人。我们试图研究真核翻译起始因子 3 亚基 b (EIF3B) 在前列腺癌中的表达和贡献。在人类前列腺患者组织和前列腺癌细胞系中分析了 EIF3B 的表达。将 EIF3B 的小干扰 RNA (siRNA) 敲低引入前列腺癌细胞系 PC-3 和 LNCaP,然后分别使用 MTT、细胞计数和末端脱氧核苷酸转移酶 dUTP 缺口末端标记分析检测细胞活力、增殖和凋亡。体内异种移植肿瘤小鼠模型也被用来解决 EIF3B 在肿瘤发生中的作用。最后,进行基因微阵列分析以搜索 EIF3B 敲低后差异表达的基因。EIF3B 在前列腺肿瘤组织和前列腺癌细胞系中上调。EIF3B 敲低抑制了前列腺癌细胞的活力和增殖,并促进了细胞凋亡。在体内小鼠模型中,接种 EIF3B 敲低 PC-3 细胞显示抑制异种移植肿瘤的生长。此外,基因微阵列还鉴定了可能参与前列腺癌中 EIF3B 作用的潜在信号通路。EIF3B 是一种新的体外和体内前列腺癌致癌因子,可作为治疗前列腺癌的新治疗靶点。接种 EIF3B 敲低的 PC-3 细胞显示异种移植肿瘤的生长受到抑制。此外,基因微阵列还鉴定了可能参与前列腺癌中 EIF3B 作用的潜在信号通路。EIF3B 是一种新的体外和体内前列腺癌致癌因子,可作为治疗前列腺癌的新治疗靶点。接种 EIF3B 敲低的 PC-3 细胞显示异种移植肿瘤的生长受到抑制。此外,基因微阵列还鉴定了可能参与前列腺癌中 EIF3B 作用的潜在信号通路。EIF3B 是一种新的体外和体内前列腺癌致癌因子,可作为治疗前列腺癌的新治疗靶点。

更新日期:2020-06-18
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