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Fungicidal activity of secondary metabolites from Annulohypoxylon sp. against the rice blast fungus, Pyricularia oryzae
Journal of General Plant Pathology ( IF 1.2 ) Pub Date : 2020-06-18 , DOI: 10.1007/s10327-020-00936-0
Yoshiya Moriguchi , Junichi Kihara , Makoto Ueno

Rice blast, a devastating disease caused by the fungus Pyricularia oryzae, causes major losses to rice production, but it continuously evolves resistance against fungicides. Therefore, natural compounds need to be identified to develop new agents to control fungal pathogens. An ethyl acetate extract of a culture filtrate (S712-ECF) from isolate S712, a member of Polyporaceae collected from a paddy field in Shimane Prefecture in 2009, was sprayed on rice leaves 24 h before inoculation with conidia of P. oryzae. S712-ECF significantly suppressed blast lesion formation on leaves. Analysis of the internal transcribed spacer (ITS)-5.8S rDNA sequence of S712 showed that it shared high similarities with species of the fungus Annulohypoxylon. S712-ECF significantly inhibited in vitro conidial germination of P. oryzae in a dose-dependent manner and had a fungicidal effect against P. oryzae. Growth inhibition zones of P. oryzae were detected in thin layer chromatography assays of S712-ECF. The most effective compound in S712-ECF was heat-stable with a molecular weight < 3000 Da. Secondary metabolites of isolate S712 thus have potential for developing a new fungicidal agent against P. oryzae.

中文翻译:

环丙氧梭菌次生代谢产物的杀菌活性。对抗稻瘟病菌Pyricularia oryzae

稻瘟病是一种由稻瘟病菌引起的毁灭性疾病,对稻米生产造成重大损失,但不断发展成对杀真菌剂的抗性。因此,需要鉴定天然化合物以开发新的药物来控制真菌病原体。在接种稻瘟病分生孢子之前24小时,将来自分离物S712的培养物滤液的乙酸乙酯提取物(S712-ECF)喷洒到水稻叶片上,分离物S712是2009年从岛根县水田采集的Poly科的一种。S712-ECF显着抑制了叶片上胚芽病变的形成。S712的内部转录间隔区(ITS)-5.8S rDNA序列分析表明,它与真菌Anhylohypoxylon的种类具有高度相似性。S712-ECF显著抑制体外孢子萌发的P.菌以剂量依赖的方式和具有针对一个杀真菌效果P.米曲霉。在S712-ECF的薄层色谱分析中检测到米曲霉的生长抑制区。S712-ECF中最有效的化合物是热稳定的,分子量<3000 Da。分离株S712的次生代谢产物因此有可能开发一种新的抗米霉菌的杀菌剂。
更新日期:2020-06-18
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