The presence of antineoplastic compounds in aquatic ecosystem is an emerging challenge for the society. Antineoplastic compounds released into the aquatic environment exhibit a potential threat to normal aquatic life. Particularly, antineoplastic compounds are responsible for direct or indirect interference with the cellular DNA of an organism and cause toxicity to cells. The present study focused on the assessment of in vitro toxic effect of cyclophosphamide, etoposide and paclitaxel on Raw 264.7 cell line (mouse monocyte macrophage cells). The inhibitory concentration of cyclophosphamide, etoposide, and paclitaxel was determined. The IC₅₀ values of these compounds were 145.44, 5.40, and 69.76 µg ml⁻¹ respectively. This is the first report on toxicity analysis of cyclophosphamide, paclitaxel and etoposide on Raw 264.7 cell line by reducing cell viability and indicating the cell cytotoxicity i.e., 69.58% for cyclophosphamide, 92.01% for etoposide and 88.85% for paclitaxel on concentration 250 µg ml⁻¹. The results of their cytotoxicity assessment highlight the need of improvement in sewage treatment technology for the efficient removal of these compounds from aquatic environment.

水生生态系统中抗肿瘤化合物的存在是社会面临的新挑战。释放到水生环境中的抗肿瘤化合物对正常的水生生物构成潜在威胁。特别地，抗肿瘤化合物负责直接或间接干扰生物体的细胞DNA并对细胞造成毒性。本研究的重点是评估环磷酰胺、依托泊苷和紫杉醇对 Raw 264.7 细胞系（小鼠单核细胞巨噬细胞）的体外毒性作用。测定环磷酰胺、依托泊苷和紫杉醇的抑制浓度。这些化合物的 IC ₅₀值为 145.44、5.40 和 69.76 µg ml ^-1分别。这是通过降低细胞活力和指示该细胞的细胞毒性，即，69.58％环磷酰胺，依托泊苷92.01％和对浓度250微克mL的紫杉醇88.85％对环磷酰胺，紫杉醇和依托泊苷上的RAW 264.7细胞系的毒性分析所述第一报告^{- 1} . 他们的细胞毒性评估结果突出表明需要改进污水处理技术，以有效地从水生环境中去除这些化合物。