A bovine colostrum fraction (BCF) was recently shown to enhance the adherence of several commensal organisms to intestinal epithelial cells through modulating the epithelial cell surface. In this study, the main components of the BCF were examined to investigate the active component/s responsible for driving the changes in the intestinal cells. The adherence of various bifidobacteria to HT-29 cells was increased when the intestinal cells were pre-incubated with immunoglobulin G (IgG). Modulation of the intestinal cells by IgG was concentration dependent with 16 mg/mL IgG resulting in a 43-fold increase in the adhesion of Bifidobacterium longum NCIMB 8809 to HT-29 cells. Periodate treatment of colostral IgG prior to performing the colonization studies resulted in a reduction in the adhesion of the strain to the intestinal cells demonstrating that the glycans of IgG may be important in modulating the intestinal cells for enhanced commensal adhesion. IgG isolated from mature milk also resulted in significant increases in adhesion of the Bifidobacterium strains tested albeit at reduced levels (3.9-fold). The impact of IgG on the HT-29 cells was also visualised via scanning electron microscopy. This study builds a strong case for the inclusion of IgG ingredients sourced from cow’s milk in functional foods aimed at increasing numbers of health promoting bacteria in the human gut.

最近显示，牛初乳馏分（BCF）通过调节上皮细胞表面来增强几种共生生物对肠上皮细胞的粘附。在这项研究中，检查了BCF的主要成分，以研究负责驱动肠道细胞变化的活性成分。当肠道细胞与免疫球蛋白G（IgG）预温育时，各种双歧杆菌对HT-29细胞的粘附增加。IgG对肠道细胞的调节与16 mg / mL IgG的浓度有关，导致长双歧杆菌粘附增加43倍NCIMB 8809至HT-29细胞。在进行定植研究之前，对初乳IgG进行定期处理会导致菌株与肠道细胞的粘附力降低，这表明IgG的聚糖可能在调节肠道细胞以增强共生粘附方面很重要。从成熟牛奶中分离出的IgG也导致测试的双歧杆菌菌株的粘附力显着增加，尽管含量降低（3.9倍）。IgG对HT-29细胞的影响也可以通过扫描电子显微镜观察。这项研究为将牛乳中的IgG成分包含在功能食品中提供了有力的依据，这些食品旨在增加人体肠道中促进健康的细菌的数量。