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Ultrastructure of the fertilized egg envelopes in Ancistrus cirrhosus, Loricariidae, Teleostei
Applied Microscopy Pub Date : 2020-06-17 , DOI: 10.1186/s42649-020-00034-7
Dong Heui Kim

We examined the morphology of fertilized egg and ultrastructures of fertilized egg envelopes of Ancistrus cirrhosus belong to Loricariidae using light and electron microscopes. The fertilized eggs formed a mass on the spawning place and were yellowish, spherical, non-transparent, demersal, adhesive, and a narrow perivitelline space. But, the adhesiveness of fertilized eggs was disappeared after spawning excluding contact parts. The micropyle with funnel shape was surrounded by 15–19 furrow lines of egg envelope in a spoke-like pattern. The outer surface of egg envelope has smooth side and inner surface of egg envelope was rough with grooves. Also, the total thickness of the fertilized egg envelope was about 32.58 ± 0.85 μm (n = 20), and the fertilized egg envelope consisted of three layers, an outer adhesive electron-dense layer, a middle layer with low electron density and an inner electron-dense layer with grooves in counter structure from other most teleost. Collectively, these morphological characteristics and adhesive property of fertilized egg, and ultrastructures of micropyle, outer surface, and section of fertilized egg envelope are showed species specificity.

中文翻译:

Ancistrus cirrhosus, Loricariidae, Teleostei 受精卵包膜的超微结构

我们使用光学和电子显微镜检查了属于 Loricariidae 的 Ancistrus cirrhosus 的受精卵的形态和受精卵包膜的超微结构。受精卵在产卵处形成团块,呈淡黄色、球形、不透明、底层、粘连、卵周间隙狭窄。但是,受精卵除接触部位外,在产卵后粘性消失。漏斗状的微孔被 15-19 条沟纹的卵包膜包围,呈辐条状。蛋壳外表面光滑,蛋壳内表面粗糙,有凹槽。此外,受精卵壳的总厚度约为 32.58 ± 0.85 μm(n = 20),受精卵壳由三层组成,外粘电子致密层,一个低电子密度的中间层和一个内部电子密集层,在其他大多数硬骨鱼的反结构中带有凹槽。总的来说,受精卵的这些形态特征和粘附特性,以及受精卵壳的微孔、外表面和截面的超微结构显示出物种特异性。
更新日期:2020-06-17
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