It may be impossible to perform cancer surgery with free margins in the presence of an unresectable structure. Local drug treatment after surgery has been proposed to increase the rate of tumor control. Multi-nanolayers (10-330 nm) were generated by a low-pressure (375mTorr) inductively coupled plasma (13.56 MHz) reactor for anticancer drug delivery by the deposition of polycaprolactone-polyethylene glycol multistack barrier on the collagen membrane (100 μm thickness). Carboplatin (300 μg/cm2) was used for the in vitro and in vivo investigations. Energy-dispersive X-ray spectroscopy (15 keV), scanning electron microscopy and inductively coupled plasma mass spectrometry were used to detect the presence of carboplatin in the nanolayer, the tumor sample and the culture medium. Preclinical studies were performed on ovarian (OVCAR-3NIH) and colon (CT26) cancer cell lines as xenografts (45 days) and allografts (23 days) in Swiss-nude (n = 6) and immunocompetent BALB/cByJ mice (n = 24), respectively. The loading of carboplatin or other drugs between the nanofilm on the collagen membrane did not modify the mesh complex architecture or the drug properties. Drugs were detectable on the membrane for more than 2 weeks in the in vitro analysis and more than 10 days in the in vivo analysis. Cytotoxic mesh decreased cell adherence (down 5.42-fold) and induced cancer cell destruction (up to 7.87-fold). Implantation of the mesh on the mouse tumor nodule modified the cell architecture and decreased the tumor size (50.26%) compared to the control by inducing cell apoptosis. Plasma technology allows a mesh to be built with multi-nanolayer anticancer drug delivery on collagen membranes.

中文翻译：

---

使用射频等离子体技术进行多纳米层药物递送。

在存在不可切除的结构的情况下，可能无法以自由边缘进行癌症手术。已提出手术后的局部药物治疗以增加肿瘤控制率。低压（375mTorr）电感耦合等离子体（13.56 MHz）反应器生成多层纳米层（10-330 nm），用于通过在胶原膜（厚度为100μm的厚度）上沉积聚己内酯-聚乙二醇多层叠层屏障来抗癌药物的递送。 。卡铂（300μg/ cm2）用于体外和体内研究。使用能量分散X射线光谱法（15 keV），扫描电子显微镜和电感耦合等离子体质谱法检测纳米层，肿瘤样品和培养基中卡铂的存在。在瑞士裸鼠（n = 6）和具有免疫功能的BALB / cByJ小鼠（n = 24）中，对卵巢（OVCAR-3NIH）和结肠（CT26）癌细胞系进行了异种移植（45天）和同种异体移植（23天）临床前研究。 ）， 分别。胶原蛋白膜上的纳米膜之间卡铂或其他药物的装载量不会改变网状复合物的结构或药物性质。在体外分析中，在膜上可检测到的药物超过2周，而在体内分析中，则在10天以上。细胞毒性网片可降低细胞粘附性（下降5.42倍）并诱导癌细胞破坏（高达7.87倍）。通过诱导细胞凋亡，与对照组相比，在小鼠肿瘤结节上植入网孔改变了细胞结构并减小了肿瘤大小（50.26％）。