Specific recognition and sensing of glycoproteins are of great importance in clinical diagnostics considering their frequent utilization as biomarkers and therapeutic targets. In this work, a biomimetic fluorescent sensor for the selective and sensitive detection of glycoprotein was developed, which was based on late-model boronate fluorescent molecularly imprinted nanoparticles (B-FMIP NPs). The B-FMIP NPs were fabricated via the macromolecular assembly of a fluorescent photo-crosslinkable amphiphilic copolymer containing boronic acid with glycoprotein in aqueous solution and in situ photo-crosslinking. Due to the synergism of boronate affinity and the molecular imprinting effect, the resultant B-FMIP NPs demonstrated specific recognition and remarkable selectivity toward the template glycoprotein (ovalbumin, OVA) with a high imprinted factor (α) of 6.0 and gave rise to obvious fluorescence quenching after binding with OVA in water. Under optimized experimental conditions, the as-prepared B-FMIP NPs exhibited linearity over the OVA concentration range of 10⁻¹³ to 10⁻³ mg mL⁻¹ with a detection limit of 3.3 × 10⁻¹⁴ mg mL⁻¹, as well as a rapid response time (about 10 min), which was superior to that of other previously reported OVA sensors. Finally, these B-FMIP NPs have been applied for the determination of OVA in real samples.

中文翻译：

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具有硼酸酯亲和力的荧光分子印迹纳米颗粒，用于选择性糖蛋白检测。

考虑到糖蛋白作为生物标志物和治疗靶标的频繁使用，糖蛋白的特异性识别和传感在临床诊断中非常重要。在这项工作中，开发了一种仿生荧光传感器，用于选择性和灵敏地检测糖蛋白，该传感器基于晚期模型的硼酸酯荧光分子印迹纳米颗粒（B-FMIP NPs）。B-FMIP NPs是通过在水溶液中原位包含硼酸和糖蛋白的荧光可光交联两亲共聚物的大分子组装而制备的光交联。由于硼酸酯亲和力和分子印迹效应的协同作用，所得的B-FMIP NPs对模板糖蛋白（卵清蛋白，OVA）具有高的印迹因子（α）为6.0的特异性识别和显着选择性，并产生明显的荧光在水中与OVA结合后淬灭。在优化的实验条件下，制备的B-FMIP NP在10 ^-13至10 ^-3 mg mL ^-1的OVA浓度范围内表现出线性，检出限为3.3×10 ^-14 mg mL ^-1，以及快速的响应时间（约10分钟），优于之前报道的其他OVA传感器的响应时间。最后，这些B-FMIP NP已用于测定真实样品中的OVA。