This study demonstrates that Thelephora ganbajun had a strong ability to absorb zinc, and zinc can be compartmentally stored in the small vesicles and mainly accumulated in the form of zinc-enriched polysaccharides (zinc content was ). Mycelia zinc polysaccharides (MZPS) and its fractions were isolated. The main fraction (MZPS-2) with the highest antioxidant activity in vitro was composed of mannose : galacturonic acid : glucose : galactose in a molar ratio of 61.19 : 1 : 39.67 : 48.67, with a weight-averaged molecular weight of . MZPS-2 had both α-pyranose and β-pyranose configuration and had a triple helical conformation. By establishing zebrafish models, we found that MZPS-2 can significantly scavenge free radicals, reduce the generation of reactive oxygen species caused by inflammation, and inhibit the recruitment of neutrophils toward the injury site. Therefore, MZPS-2 exhibited antioxidant and anti-inflammatory effects and can be used as a zinc supplement with specific biological activities to alleviate zinc deficiency complications, such as chronic oxidative stress or inflammation.

中文翻译：

---

锌在菌丝体细胞中的分布以及甘蓝特里氏菌TG-01的菌丝体锌多糖的抗氧化和抗炎活性。

这项研究表明，Thelephora ganbajun具有很强的吸收锌的能力，并且锌可以在小囊泡中间隔存储，并且主要以富锌多糖的形式积累（锌含量为）。分离了菌丝体锌多糖（MZPS）及其馏分。体外具有最高抗氧化活性的主要部分（MZPS-2）由甘露糖：半乳糖醛酸：葡萄糖：半乳糖组成，摩尔比为61.19：1：39.67：48.67，重均分子量为。MZPS-2具有α-吡喃糖和β-吡喃糖构型并且具有三重螺旋构象。通过建立斑马鱼模型，我们发现MZPS-2可以显着清除自由基，减少由炎症引起的活性氧的生成，并抑制嗜中性粒细胞向损伤部位的募集。因此，MZPS-2表现出抗氧化和抗炎作用，可以用作具有特定生物活性的锌补充剂，以缓解锌缺乏症并发症，例如慢性氧化应激或炎症。