Mutation and recombination are the primary sources of genetic variation. To better understand the evolution of genetic variation, it is crucial to comprehensively investigate the processes involving mutation accumulation and recombination. In this study, we performed mutation accumulation experiments on four heterozygous diploid yeast species in the Saccharomycodaceae family to determine spontaneous mutation rates, mutation spectra, and losses of heterozygosity (LOH). We observed substantial variation in mutation rates and mutation spectra. We also observed high LOH rates (1.65–11.07×10⁻⁶ events per heterozygous site per cell division). Biases in spontaneous mutation and LOH together with selection ultimately shape the variable genome-wide nucleotide landscape in yeast species.

中文翻译：

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酵母杂合子基因组中的可变自发突变和杂合子的丧失。

突变和重组是遗传变异的主要来源。为了更好地了解遗传变异的演变，全面研究涉及突变积累和重组的过程至关重要。在这项研究中，我们对酿酒酵母科中的四个杂合二倍体酵母物种进行了突变积累实验，以确定自发突变率，突变谱和杂合度损失（LOH）。我们观察到突变率和突变谱有很大变化。我们还观察到较高的LOH发生率（每个细胞分裂每个杂合位点为1.65-11.17×10 ^-6个事件）。自发突变和LOH中的偏向酶以及选择最终形成了酵母物种中可变的全基因组核苷酸景观。