LncRNA PlncRNA-1(PlncRNA-1) participates in breast cancer by upregulating TGF-β1. It is known that TGF-β1 plays an inhibitory role in the inflammatory responses in rheumatoid arthritis (RA). Therefore, PlncRNA-1 may also participate in RA. Serum and synovial fibroblasts were obtained from 34 patients with active RA (persistent symptoms), 36 patients with inactive RA (long term of no or few symptoms after active RA) and 40 healthy controls. Expression levels of PlncRNA-1 and TGF-β1 in active RA patients, inactive RA patients and healthy controls were measured by RT-qPCR and ELISA, respectively. Pearson Correlation Coefficient was used to determine the correlation between the expression levels of PlncRNA-1 and TGF-β1. Diagnostic value of PlncRNA-1 for active RA was detected by ROC curve analysis. PlncRNA-1 and TGF-β1 were downregulated in serum of active RA patients but not in inactive RA patients compared to the healthy controls. Expression of PlncRNA-1 and TGF-β1 were positively correlated only in RA patients, and altered expression levels of PlncRNA-1 distinguished the active RA patients from inactive RA patients and healthy controls. PlncRNA-1 and TGF-β1 were also downregulated in synovial fibroblasts derived from RA patients in comparison to inactive RA patients and healthy controls. Overexpression of PlncRNA-1 mediated upregulation of TGF-β1 in synovial fibroblasts derived from RA patients, while exogenous TGF-β1 treatment showed no significant effect on the expression of PlncRNA-1. Therefore, PlncRNA-1 participated in RA possibly by regulating TGF-β1.

LncRNA PlncRNA-1（PlncRNA-1）通过上调TGF-β1参与乳腺癌。已知TGF-β1在类风湿性关节炎（RA）的炎症反应中起抑制作用。因此，PlncRNA-1也可能参与RA。血清和滑膜成纤维细胞分别来自34例活动性RA患者（持续症状），36例非活动性RA患者（长期活动性RA后无症状或很少出现症状）和40名健康对照者。分别通过RT-qPCR和ELISA检测活跃RA患者，非活跃RA患者和健康对照者PlncRNA-1和TGF-β1的表达水平。用Pearson相关系数确定PlncRNA-1和TGF-β1表达水平之间的相关性。通过ROC曲线分析检测PlncRNA-1对活动型RA的诊断价值。与健康对照组相比，活动型RA患者的血清中PlncRNA-1和TGF-β1下调，而非活动性RA患者的血清中PlncRNA-1和TGF-β1下调。PlncRNA-1和TGF-β1的表达仅在RA患者中呈正相关，并且PlncRNA-1表达水平的改变将活跃RA患者与非活跃RA患者和健康对照区分开。与非活动性RA患者和健康对照组相比，RA患者的滑膜成纤维细胞中PlncRNA-1和TGF-β1也下调。RA患者滑膜成纤维细胞中PlncRNA-1的过表达介导TGF-β1的上调，而外源性TGF-β1的治疗对PlncRNA-1的表达无明显影响。因此，PlncRNA-1可能通过调节TGF-β1参与了RA。PlncRNA-1和TGF-β1的表达仅在RA患者中呈正相关，并且PlncRNA-1表达水平的改变将活跃RA患者与非活跃RA患者和健康对照区分开。与非活动性RA患者和健康对照组相比，RA患者的滑膜成纤维细胞中PlncRNA-1和TGF-β1也下调。RA患者滑膜成纤维细胞中PlncRNA-1的过表达介导TGF-β1的上调，而外源性TGF-β1处理对PlncRNA-1的表达无明显影响。因此，PlncRNA-1可能通过调节TGF-β1参与了RA。PlncRNA-1和TGF-β1的表达仅在RA患者中呈正相关，并且PlncRNA-1表达水平的改变将活跃RA患者与非活跃RA患者和健康对照区分开。与非活动性RA患者和健康对照组相比，RA患者的滑膜成纤维细胞中PlncRNA-1和TGF-β1也下调。RA患者滑膜成纤维细胞中PlncRNA-1的过表达介导TGF-β1的上调，而外源性TGF-β1处理对PlncRNA-1的表达无明显影响。因此，PlncRNA-1可能通过调节TGF-β1参与了RA。与非活动性RA患者和健康对照组相比，RA患者的滑膜成纤维细胞中PlncRNA-1和TGF-β1也下调。RA患者滑膜成纤维细胞中PlncRNA-1的过表达介导TGF-β1的上调，而外源性TGF-β1的治疗对PlncRNA-1的表达无明显影响。因此，PlncRNA-1可能通过调节TGF-β1参与了RA。与非活动性RA患者和健康对照组相比，RA患者的滑膜成纤维细胞中PlncRNA-1和TGF-β1也下调。RA患者滑膜成纤维细胞中PlncRNA-1的过表达介导TGF-β1的上调，而外源性TGF-β1的治疗对PlncRNA-1的表达无明显影响。因此，PlncRNA-1可能通过调节TGF-β1参与了RA。