Silk fibroin (SF) microparticles were fabricated by blending of SF solution with hydroxyl propyl methyl cellulose (HPMC), and the SF/HPMC blend films were prepared using simple solvent evaporation technique. The SF/HPMC blends were dissolved in ultrapure water, and SF microparticles were generated with and without sonication. Fourier transformation infrared spectroscopy (FTIR), scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA)/derivative thermogravimetric (DTG) measurements were used for the characterization of blend films and microparticles. The microparticles obtained sonicated consist of nanosized pores on the surface. The size of the pores was nano, but the diameter of particles was around 10 µm. Further, SF microparticles were evaluated as a novel biosorbent for the methylene blue (MB), a cationic dye. The influential factors (pH, contact time) to the adsorption of the dye by the microparticles were investigated. The SF microparticles showed a very good adsorption capacity of about 490 (nonsonicated) and of about 512 (sonicated) mg/g of the dye without any surfactants. The adsorption kinetics validates the practicality of using the pseudo-second-order kinetics. Langmuir and Freundlich isothermal models were used, and the results showed that the adsorption behavior of the samples best fits (R²=0.998) the Langmuir model. The easy preparation and obtained results suggest that the SF microparticles can be used as effective adsorbent for MB dye removal.

通过将SF溶液与羟丙基甲基纤维素（HPMC）共混制备丝素蛋白（SF）微粒，并使用简单的溶剂蒸发技术制备SF / HPMC共混膜。将SF / HPMC共混物溶解在超纯水中，并在进行超声处理和不进行超声处理的情况下生成SF微粒。傅立叶变换红外光谱（FTIR），扫描电子显微镜（SEM），差示扫描量热法（DSC）和热重分析（TGA）/衍生热重（DTG）测量用于表征共混膜和微粒。超声处理所得的微粒由表面上的纳米孔组成。孔的尺寸为纳米，但是颗粒的直径为约10μm。进一步，SF微粒被评估为亚甲基蓝（MB）（一种阳离子染料）的新型生物吸附剂。研究了微粒对染料吸附的影响因素（pH，接触时间）。SF微粒在没有任何表面活性剂的情况下显示出约490（非超声）和512（超声）mg / g染料的非常好的吸附能力。吸附动力学验证了使用伪二级动力学的实用性。使用Langmuir和Freundlich等温模型，结果表明样品的吸附行为最适合（R SF微粒在没有任何表面活性剂的情况下显示出约490（未超声）和512（超声）mg / g染料的非常好的吸附能力。吸附动力学验证了使用伪二级动力学的实用性。使用Langmuir和Freundlich等温模型，结果表明样品的吸附行为最适合（R SF微粒在没有任何表面活性剂的情况下显示出约490（未超声）和512（超声）mg / g染料的非常好的吸附能力。吸附动力学验证了使用伪二级动力学的实用性。使用Langmuir和Freundlich等温模型，结果表明样品的吸附行为最适合（R² = 0.998）Langmuir模型。易于制备和获得的结果表明，SF微粒可用作MB染料去除的有效吸附剂。