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Effects of anti-wrinkle and skin-whitening fermented black ginseng on human subjects and underlying mechanism of action.
Journal of Toxicology and Environmental Health, Part A ( IF 2.6 ) Pub Date : 2020-06-16 , DOI: 10.1080/15287394.2020.1776454
Jin Ju Park 1 , Junmin An 2 , Jung Dae Lee 1 , Hyang Yeon Kim 1 , Jueng Eun Im 1 , Eunyoung Lee 3 , Jaehyoun Ha 3 , Chang Hui Cho 3 , Dong-Wan Seo 1 , Kyu-Bong Kim 1
Affiliation  

The aim of this study was to determine the effects of anti-wrinkle and skin-whitening of fermented black ginseng (FBG) in human subjects and to examine underlying biochemical mechanisms of action. A clinical study was performed to evaluate efficacy and safety using a 1% FBG cream formulation. Twenty-three subjects were recruited and instructed to apply control or FBG creams each on half of their face twice daily for 8 weeks. After 8 weeks FBG cream significantly reduced appearance of eye wrinkles compared to prior to exposure and control cream. Skin color was significantly brightened using FBG cream in comparison with control cream. To determine the mechanism of actions involved in anti-wrinkle and skin-whitening effects various concentrations of FBG were applied to human fibroblast CCD-986sk and mouse melanoma B16F1 cells. Collagen synthesis in CCD-986sk cells was improved significantly at 1, 3, 10, or 30 µg/ml of FBG. At 30 µg/ml, FBG significantly inhibited (73%) collagenase, and matrix metalloproteinase-1 (MMP-1) compared to control. Tyrosinase activity and DOPA (3,4-dihydroxy-L-phenylalanine) oxidation were significantly decreased at all tested concentrations. Melanin production in B16F1 cells was concentration-dependently reduced 15% to 60% by all concentrations of FBG. These results suggested that a 1% FBG cream exerted anti-wrinkle and skin-whitening effects.



中文翻译:

抗皱和美白发酵黑参对人体的作用及其潜在的作用机理。

这项研究的目的是确定发酵黑参(FBG)在人类受试者中的抗皱和美白皮肤的作用,并研究其潜在的生化作用机理。使用1%FBG乳膏制剂进行了一项临床研究,以评估疗效和安全性。招募了二十三名受试者,并指示他们分别在其一半的脸部上分别使用控制霜或FBG霜两次,连续8周。与暴露和对照乳霜之前相比,FBG乳霜8周后,眼皱纹的出现明显减少。与对照乳霜相比,使用FBG乳霜可显着提亮肤色。为了确定抗皱和美白作用的作用机理,将各种浓度的FBG应用于人成纤维细胞CCD-986sk和小鼠黑素瘤B16F1细胞。在FBG浓度为1、3、10或30μg/ ml时,CCD-986sk细胞中的胶原蛋白合成显着改善。与对照组相比,FBG以30 µg / ml的浓度可显着抑制(73%)胶原酶和基质金属蛋白酶-1(MMP-1)。在所有测试浓度下,酪氨酸酶活性和DOPA(3,4-二羟基-L-苯丙氨酸)氧化均显着降低。通过所有浓度的FBG,B16F1细胞中黑色素的生成浓度依赖性地降低了15%至60%。这些结果表明,1%FBG乳霜具有抗皱和美白皮肤的作用。在所有测试浓度下,4-二羟基-L-苯丙氨酸的氧化均显着降低。通过所有浓度的FBG,B16F1细胞中黑色素的生成浓度依赖性地降低了15%至60%。这些结果表明,1%FBG乳霜具有抗皱和美白皮肤的作用。在所有测试浓度下,4-二羟基-L-苯丙氨酸的氧化均显着降低。通过所有浓度的FBG,B16F1细胞中黑色素的生成浓度依赖性地降低了15%至60%。这些结果表明,1%FBG乳霜具有抗皱和美白皮肤的作用。

更新日期:2020-06-22
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