Generating a biofunctional film that can mimic the extracellular matrix (ECM) in an efficient and robust technique that may have great potential for medical devices, tissue engineering, and regenerative medicines. Herein, a facile approach to generate ECM biomimetic films based on the humidity‐triggered relaxation of polyelectrolyte complex (PEC) nanoparticles is reported. The poly(l ‐lysine) and hyaluronan are precomplexed and sprayed onto a substrate, which, via a trigger of vaporous water, can be transformed into an even and stable film. The spontaneous polymer chain interfusion (diffusion coefficient ≈1.01 × 10⁻⁹ cm² s⁻¹) under saturated humidity, allowing for the rapid reorganization (within 30 min) of film morphology and structure is demonstrated. A controllable and scalable way for the loading of diversified bioactive agents, as well as on‐demand modulation of stiffness is further presented. Moreover, the high‐throughput arrays and programmed patterns can be easily completed, suggesting huge potentials that surpass those of state‐of‐the‐art methods. Combined with high efficiency and flexible functionalization, it is believed that this approach should be beneficial for extending the practical applications of PEC films, such as medical implants, chip detectors, and so on.

中文翻译：

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湿气引发的聚电解质复合物弛豫是产生细胞外基质仿生膜的稳健方法。

生成一种可以模仿细胞外基质（ECM）的生物功能膜，该膜可以用于医疗设备，组织工程和再生医学，具有很高的潜力。本文中，报道了一种基于湿度触发的聚电解质复合物（PEC）纳米颗粒松弛产生ECM仿生膜的简便方法。将聚（l-赖氨酸）和透明质酸预混合并喷涂到基材上，通过触发水蒸气，可以将其转化为均匀而稳定的薄膜。自发的聚合物链互穿（扩散系数≈1.01×10 ^-9  cm ²  s ^-1）在饱和湿度下，可以使薄膜的形态和结构迅速重组（在30分钟内）。进一步提出了一种可控和可扩展的方式来加载多种生物活性剂，以及按需调节硬度。此外，高通量阵列和编程模式可以轻松完成，这表明其潜力已超过最新方法。结合高效和灵活的功能化，相信这种方法应该有益于扩展PEC膜的实际应用，例如医疗植入物，芯片检测器等。