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Anti-inflammatory effect of selenium on lead-induced testicular inflammation by inhibiting NLRP3 inflammasome activation in chickens
Theriogenology ( IF 2.8 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.theriogenology.2020.06.015
He Huang 1 , Xiaoyu Li 1 , Ziming Wang 1 , Xu Lin 1 , Yaguang Tian 1 , Qian Zhao 1 , Peng Zheng 1
Affiliation  

Lead (Pb) is a deleterious environmental pollutant that is toxic to testes. Selenium (Se) possesses antioxidant and anti-inflammatory properties. Nucleotide-binding domain, leucine-rich-containing family, pyrin-domain containing-3 (NLRP3) inflammasome is involved in inflammatory response. However, the function of NLRP3 inflammasome in antagonistic effect of Se on inflammation caused by Pb remains unknown. The purpose of this research is to identify anti-inflammatory role of Se on testicular toxicity induced by Pb with an emphasis on oxidative stress, inflammation and NLRP3 signaling pathway in chicken. In present study, sixty seven-day-old Hyline male chickens were assigned into four groups. The feeding program consisted of a commercial diet (0.49 mg/kg Se), a Se-supplemented diet (1 mg/kg Se), a Pb-supplemented diet (0.49 mg/kg Se and 350 mg/kg Pb) and a Se-supplemented and Pb-supplemented diet (1 mg/kg Se and 350 mg/kg Pb), respectively. On the 12th week, blood was collected to measure serum testosterone level and testicular tissues were removed to determine Se and Pb concentrations, testicular function, histological structure, oxidative stress indicators and inflammation-related factors (Nuclear factor-kappaB, tumor necrosis factor-α, cyclooxygenase-2, NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain, caspase-1, interluekin (IL)-1β, IL-6, IL-18 and interferon-γ). The experimental results showed that after Pb administration, testicular injury was confirmed via histological assessment; testicular dysfunction were further indicated by decreased testosterone level and mRNA expression of steroidogenic acute regulatory protein, cytochrome P450 side-chain cleavage, 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase. Moreover, NLRP3 signaling pathway activated by Pb-caused oxidative stress was up-regulated accompanied by promotion in reactive oxygen species, nitric oxide, inducible nitric oxide synthase and malondialdehyde and reduction in antioxidants including glutathione peroxidase and glutathione s-transferase. Se administration ameliorated testicular tissue injury, testicular function, oxidative stress and inflammation. In conclusion, Se exhibited antagonistic role in Pb-induced testicular injury via enhancing antioxidant system and inhibiting inflammation in chickens.

中文翻译:

硒通过抑制 NLRP3 炎性体激活对铅致睾丸炎症的抗炎作用

铅 (Pb) 是一种有害的环境污染物,对睾丸有毒。硒 (Se) 具有抗氧化和抗炎特性。核苷酸结合域、富含亮氨酸的家族、含有 pyrin 域的 3 (NLRP3) 炎症小体参与炎症反应。然而,NLRP3炎症小体在Se对Pb引起的炎症的拮抗作用中的作用尚不清楚。本研究的目的是确定硒对 Pb 诱导的睾丸毒性的抗炎作用,重点是鸡的氧化应激、炎症和 NLRP3 信号通路。在本研究中,将 67 天大的 Hyline 雄性鸡分为四组。喂养计划包括商业饮食(0.49 mg/kg Se)、补充硒饮食(1 mg/kg Se)、补充铅饮食(0. 49 mg/kg Se 和 350 mg/kg Pb)和补充硒和补充铅的饮食(1 mg/kg Se 和 350 mg/kg Pb)。第12周采血测定血清睾酮水平,切除睾丸组织测定Se、Pb浓度、睾丸功能、组织结构、氧化应激指标和炎症相关因子(核因子-kappaB、肿瘤坏死因子-α) 、环氧合酶-2、NLRP3、包含半胱天冬酶募集结构域的凋亡相关斑点样蛋白、半胱天冬酶-1、白介素 (IL)-1β、IL-6、IL-18 和干扰素-γ)。实验结果表明,Pb给药后,经组织学评估证实睾丸损伤;睾丸激素水平降低和类固醇生成急性调节蛋白的 mRNA 表达进一步表明睾丸功能障碍,细胞色素 P450 侧链裂解、3β-羟基类固醇脱氢酶和 17β-羟基类固醇脱氢酶。此外,由铅引起的氧化应激激活的 NLRP3 信号通路被上调,伴随着活性氧、一氧化氮、诱导型一氧化氮合酶和丙二醛的促进以及包括谷胱甘肽过氧化物酶和谷胱甘肽 s-转移酶在内的抗氧化剂的减少。硒管理改善了睾丸组织损伤、睾丸功能、氧化应激和炎症。总之,硒通过增强抗氧化系统和抑制鸡的炎症,在铅诱导的睾丸损伤中表现出拮抗作用。由铅引起的氧化应激激活的 NLRP3 信号通路上调伴随着活性氧、一氧化氮、诱导型一氧化氮合酶和丙二醛的促进以及包括谷胱甘肽过氧化物酶和谷胱甘肽 s 转移酶在内的抗氧化剂的减少。硒管理改善了睾丸组织损伤、睾丸功能、氧化应激和炎症。总之,硒通过增强抗氧化系统和抑制鸡的炎症,在铅诱导的睾丸损伤中表现出拮抗作用。由铅引起的氧化应激激活的 NLRP3 信号通路上调伴随着活性氧、一氧化氮、诱导型一氧化氮合酶和丙二醛的促进以及包括谷胱甘肽过氧化物酶和谷胱甘肽 s 转移酶在内的抗氧化剂的减少。硒管理改善了睾丸组织损伤、睾丸功能、氧化应激和炎症。总之,硒通过增强抗氧化系统和抑制鸡的炎症,在铅诱导的睾丸损伤中表现出拮抗作用。氧化应激和炎症。总之,硒通过增强抗氧化系统和抑制鸡的炎症,在铅诱导的睾丸损伤中表现出拮抗作用。氧化应激和炎症。总之,硒通过增强抗氧化系统和抑制鸡的炎症,在铅诱导的睾丸损伤中表现出拮抗作用。
更新日期:2020-10-01
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