Surface plasmon resonance was used for the detection of different kinds of organic molecules, which was achieved employing a Kretschmann configuration. A simple theoretical model based on effective medium theory was used for estimation of the SPR angle. The employed model considers an effective refractive index, which has some advantages in comparison to other effective medium theories. This model depends on the surface coverage, size of the sample and also considers the scattering by molecules deposited on the surface of the thin film. Using an effective refractive index as a tool to predict the behavior of the SPR signal offers a significant advantage for the development of new biosensors, providing a deeper physical insight on the behavior of such phenomena, since inferring the behavior of SPR signals is of great importance for chemical analysis and bio-sensing. Therefore, in this work, an effective medium model was compared with experimental SPR measurements as a function of angle incidence of different molecules deposited onto thin films, specifically Bovine Serum Albumin (BSA) and carbamazepine attached to BSA, in order to validate the theoretical model. Evaluation of the proposed model for SPR signal was in good agreement with the experimental results. Statistical tests were obtained, with a 99% of correlation between the experimental data and the theoretical predictions around the resonance.

表面等离振子共振用于检测不同种类的有机分子，这是采用Kretschmann配置实现的。基于有效介质理论的简单理论模型用于估算SPR角。使用的模型考虑了有效折射率，与其他有效介质理论相比，它具有一些优势。该模型取决于表面覆盖率，样品尺寸，还考虑了沉积在薄膜表面上的分子的散射。使用有效折射率作为预测SPR信号行为的工具，为开发新型生物传感器提供了显着优势，从而可以更深入地了解此类现象的行为，因为推断SPR信号的行为对于化学分析和生物传感非常重要。因此，在这项工作中，将有效的介质模型与实验SPR测量值作为沉积在薄膜上的不同分子（尤其是附着于BSA的牛血清白蛋白（BSA）和卡马西平）的不同分子的入射角的函数进行了比较，以验证理论模型。对提出的SPR信号模型的评估与实验结果非常吻合。获得统计测试，实验数据与共振周围的理论预测之间具有99％的相关性。为了验证理论模型，特别是将牛血清白蛋白（BSA）和卡马西平连接到BSA。对提出的SPR信号模型的评估与实验结果非常吻合。获得统计测试，实验数据与共振周围的理论预测之间具有99％的相关性。为了验证理论模型，特别是将牛血清白蛋白（BSA）和卡马西平连接到BSA。对提出的SPR信号模型的评估与实验结果非常吻合。获得统计测试，实验数据与共振周围的理论预测之间具有99％的相关性。