This study investigated the effects of different nitrogen (N) forms on Cadmium (Cd) uptake and accumulation in dwarf Polish wheat (DPW) seedlings, which were grown under Cd stress with N-Null, NH₄⁺-N, NO₃⁻-N and NH₄⁺-N + NO₃⁻-N. We measured plant growth and determined Cd uptake, translocation, accumulation, subcellular distribution and chemical forms in the roots and shoots of DPW seedlings. We also analyzed saccharide concentrations, and the transcript levels of genes encoding metal transporters in the roots of DPW seedlings. In the absence of NO₃⁻-N, addition of NH₄⁺-N reduced roots Cd concentration, F_CW (Cd in cell wall), F_S (Cd in soluble fraction) and F_E (inorganic Cd) concentrations, and induced the expression of four genes encoding metal transporters in roots, while it promoted Cd translocation to shoots. In the presence of NO₃⁻-N, addition of NH₄⁺-N increased roots Cd concentration, F_CW and F_W concentrations, and induced the expression of 22 genes encoding metal transporters in roots. Regardless of NH₄⁺-N level, addition of NO₃⁻-N increased roots Cd concentration, F_CW, F_S, F_W (water-soluble Cd), F_NaCl (pectates and protein Cd), F_HAc (undissolved Cd phosphate) and lactose concentrations, and also induced the expression of genes encoding metal transporters in roots. Overall, NH₄⁺-N differently regulated Cd uptake and accumulation in DPW seedlings in the absence or presence of NO₃⁻-N, while NO₃⁻-N greatly increased Cd uptake and accumulation in the presence of NH₄⁺-N compared to the absence of NH₄⁺-N. These patterns of Cd alteration likely arose due to different N forms altering Cd subcellular distribution and chemical forms, lactose concentration and the expression of metal transporter genes.

本研究探讨不同的氮的影响（N）在矮波兰小麦上镉（Cd）的摄取和积累的形式（DPW）幼苗，将其镉胁迫下生长与N-空，NH ₄ ⁺ -N，NO ₃ ^- - N和NH ₄ ⁺ -N + NO ₃ ^-- N。我们测量了植物的生长，并确定了DPW幼苗根和茎中的Cd吸收，转运，积累，亚细胞分布和化学形式。我们还分析了糖的浓度，以及DPW幼苗根中编码金属转运蛋白的基因的转录水平。在没有NO ₃ ^-- N的情况下，NH ₄ ⁺ -N的添加会降低根部Cd的浓度，F_CW（Cd的细胞壁），F_小号（CD在可溶性级分）和F _È（无机镉）的浓度，和诱导的编码根金属转运四种基因的表达，而它促进镉易位至芽。在NO ₃ ^-- N存在下，NH ₄ ⁺ -N的添加增加了根的Cd浓度，F _CW和F _W浓度，并诱导了根中编码金属转运蛋白的22个基因的表达。无论NH的₄ ⁺ -N水平，此外NO的₃ ^- -N增加根Cd含量，F _CW，F_小号，F _{w ^}（水溶性Cd），F _NaCl（果胶和蛋白质Cd），F _HAc（未溶解的Cd磷酸盐）和乳糖浓度，还诱导了根中编码金属转运蛋白的基因的表达。总体而言，在不存在或存在NO ₃ ^-- N的情况下，NH ₄ ⁺ -N对DPW幼苗中Cd的吸收和积累的调控不同，而在存在NH ₄ ⁺ -N的情况下，NO ₃ ^-- N显着提高了Cd的吸收和积累。到没有NH ₄ ⁺-N。这些Cd改变的模式可能是由于不同的N形式改变了Cd的亚细胞分布和化学形式，乳糖浓度和金属转运蛋白基因的表达而引起的。