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In vitro induction and characterization of hexaploid Pennisetum × advena , an ornamental grass
Plant Cell, Tissue and Organ Culture ( IF 3 ) Pub Date : 2020-06-17 , DOI: 10.1007/s11240-020-01814-5
Yuesen Yue , Xifeng Fan , Yaofang Hu , Chao Han , Hui Li , Wenjun Teng , Hui Zhang , Ke Teng , Haifeng Wen , Xuejun Yang , Juying Wu

Pennisetum × advena (purple fountain grass) is an ornamental grass with considerable commercial value. In vitro induction of hexaploids and evaluation of the morphological and anatomical traits in P. × advena were conducted in this study. Nine independent hexaploids were generated from calli, which was initiated from immature inflorescence tissue of triploid P. × advena, treated with 0.1% colchicine for 24 h. Their ploidy levels were first screened by flow cytometry and further identified by chromosome counting. Compared with the triploids, the hexaploids showed a significant delay in flowering, and reduction in plant height, the number of tillers and number of inflorescences per plant, leaf length and width, inflorescence diameter, and the number of florets per inflorescence, but an increase in floret fresh weight, and anther diameter and length. Pollen grains in the hexaploids were well developed, and their fertility was restored. The production of hexaploids of P. × advena by colchicine treatment, and assessment of their growth parameters and pollen fertility, provide important information for future breeding of P. × advena.



中文翻译:

观赏草六倍体狼尾草的体外诱导与鉴定。

狼尾草 ×  advena(紫色喷泉草)是具有相当商业价值的观赏草。在六倍体的体外诱导,并在形态解剖性状的评价P。× advena进行了这项研究。九个独立的六倍体是从愈伤组织中产生的,而愈伤组织是由三倍体P的未成熟花序组织引发的。× advena,用0.1%秋水仙碱处理24小时。首先通过流式细胞仪筛选它们的倍性水平,然后通过染色体计数进一步鉴定。与三倍体相比,六倍体的开花显着延迟,株高,单株分till和花序数,叶长和宽,花序直径和每花序的小花数减少,但增加了。在小花的新鲜重量,以及花药的直径和长度。六倍体中的花粉粒发育良好,繁殖力得以恢复。P的六倍体的产生。×秋水仙碱处理的advena,以及对其生长参数和花粉育性的评估,为将来的P繁殖提供了重要信息。×advena

更新日期:2020-06-17
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