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Mollusk microbiota shift during Angiostrongylus cantonensis infection in the freshwater snail Biomphalaria glabrata and the terrestrial slug Phillocaulis soleiformis.
Parasitology Research ( IF 2 ) Pub Date : 2020-06-17 , DOI: 10.1007/s00436-020-06743-y
Joana Borges Osório 1 , Leandro de Mattos Pereira 1, 2 , Adriana Giongo 3 , Letícia Marconatto 1, 2, 3, 4, 5, 6 , Jeremy Potriquet 4 , Renata Russo Frasca Candido 5 , Jason Mulvenna 3 , Malcolm Jones 6 , Carlos Graeff-Teixeira 1 , Alessandra Loureiro Morassutti 1
Affiliation  

In the present work, we reported for the first time the microbiome from Phyllocaulis soleiformis and Biomphalaria glabrata assessed using high-throughput DNA sequencing pre- and post-infection with the helminth parasite Angiostrongylus cantonensis. B. glabrata and P. soleiformis were experimentally infected with A. cantonensis. Fecal DNAs from control and infected groups were extracted and subjected to 16S rRNA high-throughput sequencing survey. No significant differences were found in the alpha diversity indexes in Phyllocaulis and Biomphalaria experiments independently. PCoA analysis using the unweighted UniFrac measures showed that both microbiotas behaved differently depending on the host. In Biomphalaria microbiota, control and infected groups were significantly different (p = 0.0219), while Phyllocaulis samples were not (p = 0.5190). The microbiome of P. soleiformis infected with A. cantonensis showed a significant decrease of Sphingobacterium and a substantial increase of Cellvibrio when compared to a control group. The microbiome of B. glabrata infected with A. cantonensis showed a significant decline in the abundance of Flavobacterium, Fluviicola, Nitrospira, Vogesella and an OTU belonging to the family Comamonadaceae, and a significant increase of Uliginosibacterium and an OTU belonging to the family Weeksellaceae when compared to a control group. Overall, the microbiome data reported here provided valuable information with regard to the diversity of bacterial communities that comprise the gut microbiome of gastropods. Furthermore, we report here the effect of the infection of the helminth A. cantonensis in the ratio and distribution of the fecal microbiome of the snails. Further studies are highly valuable in order to better understand those interactions by comparing different microbiome profiles and mollusk models. By now, we anticipate that ecological studies will take significant advantage of these advances, particularly concerning improving our understanding of helminth-microbiome-host interactions.



中文翻译:

在淡水蜗牛Biomphalaria glabrata和陆地Phillocaulis soleiformis感染广州管圆线虫的过程中,软体动物的微生物群转移。

在目前的工作中,我们报道首次从微生物Phyllocaulis soleiformis贝罗光滑利用高通量DNA测序前,后感染蠕虫寄生虫评估的广州管圆线虫。实验证明光滑芽孢杆菌B. glabrata)和比目毕赤酵母P. soleiformis)广东曲霉感染。提取对照组和感染组的粪便DNA,并进行16S rRNA高通量测序调查。PhyllocaulisBiomphalaria的α多样性指数没有发现显着差异独立进行实验。使用未加权UniFrac测度的PCoA分析显示,两种微生物群的行为取决于宿主。在贝罗比微生物群,控制和受感染的组均显著不同(p  = 0.0219),而Phyllocaulis样品没有(p  = 0.5190)。与对照组相比,感染广州小曲霉的比目鱼假单胞菌的微生物组显示了鞘氨醇杆菌的显着减少和细胞弧菌的显着增加。的微生物B.光滑念珠菌感染广州管圆线虫表现出丰富的显著下降黄杆菌Fluviicola硝化Vogesella和属于家庭的OTU Comamonadaceae,和一个显著上升Uliginosibacterium和属于家庭的OTU Weeksellaceae相比对照组时。总体而言,此处报告的微生物组数据提供了有关组成腹足动物肠道微生物组的细菌群落多样性的宝贵信息。此外,我们在这里报告了感染蠕虫A. cantonensis的影响蜗牛粪便微生物组的比例和分布。为了通过比较不同的微生物组图谱和软体动物模型更好地了解这些相互作用,进一步的研究非常有价值。到目前为止,我们预计生态学研究将充分利用这些进展,特别是在提高对蠕虫-微生物组-宿主相互作用的理解方面。

更新日期:2020-06-17
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